Eph/ephrin interactions modulate vascular sympathetic innervation
Eph/ephrin interactions modulate vascular sympathetic innervation
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DOI：
10.1016/j.autneu.2010.06.004
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发表时间：
2010-12-08
影响因子：
2.7
通讯作者：
Marko, Stephen B.
中科院分区：
文献类型：
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作者：
Damon, Deborah H.;TeRiele, Jaclyn A.;Marko, Stephen B.
Eph蛋白和ephrin蛋白是膜结合蛋白，它们相互作用以调节轴突生长和神经元功能。我们检验了Eph/ephrin相互作用影响血管交感神经支配的生长和功能这一假设。通过逆转录聚合酶链反应分析，我们在新生和成年大鼠的交感神经节中检测到了两类Eph蛋白（A类和B类）以及两类ephrin蛋白（A类和B类）。两类Eph蛋白（A类和B类）以及两类ephrin蛋白（A类和B类）都结合到分离的节后交感神经元的细胞体和神经突上。在新生和成年大鼠的交感神经支配的动脉中也检测到了编码两类Eph蛋白（A类和B类）以及两类ephrin蛋白（A类和B类）的信使RNA。这些数据表明，节后交感神经元神经纤维上的ephrin/Eph蛋白可能与受神经支配的动脉中的Eph/ephrin蛋白相互作用。我们发现EphA4减少了去神经的股动脉的再神经支配。在存在EphA4 - Fc的情况下的再神经支配（38.9±6.6%）显著少于在存在IgG - Fc的情况下（62±10%，n = 5，p（此处似乎内容不完整））
Ephs and ephrins are membrane-bound proteins that Interact to modulate axon growth and neuronal function We tested the hypothesis that eph/ephrin interactions affected the growth and function of vascular sympathetic innervation Using RT PCR analyses we detected both classes of ephs (A and B) and both classes of ephrins (A and B) in sympathetic ganglia from neonatal and adult rats Both classes of ephs (A and B) and both classes of ephrins (A and B) bound to the cell bodies and neurites of dissociated postganglionic sympathetic neurons Messenger RNAs encoding for both classes of ephs (A and B) and both classes of ephrins (A and B) were also detected in sympathetically innervated arteries from neonatal and adult rats These data suggest that ephrins/ephs on nerve fibers of postganglionic sympathetic neurons could inter ict with ephs/ephrins on cells in Innervated arteries We found that ephA4 reduced reinnervation of denervated femoral arteries Reinnervation in the presence of ephA4-Fc (38 9 +/- 6 6%) was significantly less than that in the presence of IgG-Fc (62 +/- 10% n = 5 p