Compressed collagen get: a novel scaffold for human bladder cells
Compressed collagen get: a novel scaffold for human bladder cells
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DOI：
10.1002/term.222
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发表时间：
2010-03-01
影响因子：
3.3
通讯作者：
Frey, P.
中科院分区：
文献类型：
--
作者：
Engelhardt, E. -M.;Stegberg, E.;Frey, P.
胶原蛋白是高度保守的跨物种，并已广泛用于组织再生；然而，它的机械性能是有限的。最近的一项进展是利用胶原蛋白凝胶的塑料压缩来获得更高的浓度，显著提高了其在新组织水平上的机械性能。这种受控的、独立于细胞的过程使仿生支架的工程成为可能。我们已经评估了塑料压缩胶原蛋白支架内植入人膀胱平滑肌细胞，凝胶表面植入尿路上皮细胞，用于泌尿外科的潜在应用。采用扫描电镜、常规组织学和免疫组化观察膀胱平滑肌和尿路上皮细胞；体外培养14天，定量测定细胞活力和增殖能力。2周后，两种类型的细胞均在构建体表面增殖，形成致密的细胞层。然而，用Alamar蓝试验评估，在构建物中植入的平滑肌细胞增殖较低。使用共聚焦显微镜还评估了构造内的细胞分布。体外培养14天后，在构建体表面发现30%的平滑肌细胞，而第1天为0%。我们的研究结果提供了一些证据，表明细胞种子塑料压缩胶原蛋白在膀胱组织再生中具有重要的潜力，因为这些材料可以有效地在结构内部播种细胞并使细胞增殖。版权所有John Wiley & Sons， Ltd. 2009
Collagen is highly conserved across species and has been used extensively for tissue regeneration; however, its mechanical properties are limited. A recent advance using plastic compression of collagen gels to achieve much higher concentrations significantly increases its mechanical properties at the neo-tissue level. This controlled, cell-independent process allows the engineering of biomimetic scaffolds. We have evaluated plastic compressed collagen scaffolds seeded with human bladder smooth muscle cells inside and urothelial cells on the gel surface for potential urological applications. Bladder smooth muscle and urothelial cells were visualized using scanning electron microscopy, conventional histology and immunohistochemistry; cell viability and proliferation were also quantified for 14 days in vitro. Both cell types tested proliferated on the construct surface, forming dense cell layers after 2 weeks. However, smooth muscle cells seeded within the construct, assessed with the Alamar blue assay, showed lower proliferation. Cellular distribution within the construct was also evaluated, using confocal microscopy. After 14 days of in vitro culture, 30% of the smooth muscle cells were found on the construct surface compared to 0% at day 1. Our results provide some evidence that cell-seeded plastic compressed collagen has significant potential for bladder tissue regeneration, as these materials allow efficient cell seeding inside the construct as well as cell proliferation. Copyright (C) 2009 John Wiley & Sons, Ltd.