Biodesulfurization in biphasic systems containing organic solvents
Biodesulfurization in biphasic systems containing organic solvents
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DOI：
10.1128/aem.00081-06
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发表时间：
2006-07-01
影响因子：
4.4
通讯作者：
Ma, Cui Qing
中科院分区：
文献类型：
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作者：
Tao, Fei;Yu, Bo;Ma, Cui Qing
双相体系可以克服传统介质产率低的问题，并已被开发用于生物催化。耐溶剂微生物可用于在双相反应中用全细胞进行生物转化。将红平红球菌（Rhodococcus erythropolis XP）的生物降解基因簇dszABCD导入耐溶剂的恶臭假单胞菌（P. putida）爱达荷州，构建了一株耐溶剂的恶臭假单胞菌A4。采用两相反应研究了各种含硫杂环化合物在各种有机溶剂中的脱硫反应。恶臭假单胞菌A4的底物范围与R.在10%（vol/vol）对二甲苯存在下，在前2 h内可以以1.29 mM g（干重）细胞（-1）h（-1）的特定速率降解二苯并噻吩。恶臭假单胞菌A4也能够在浓度为10%（体积/体积）的许多其他有机溶剂的存在下降解二苯并噻吩。这项研究是一个重要的一步，在探索新的生物催化剂的生物技术潜力，开发一个有效的生物脱硫过程中的两相反应混合物含有有毒的有机溶剂。
Biphasic systems can overcome the problem of low productivity in conventional media and have been exploited for biocatalysis. Solvent-tolerant microorganisms are useful in biotransformation with whole cells in biphasic reactions. A solvent-tolerant desulfurizing bacterium, Pseudomonas putida A4, was constructed by introducing the biodesulfurizing gene cluster dszABCD, which was from Rhodococcus erythropolis XP, into the solvent-tolerant strain P. putida Idaho. Biphasic reactions were performed to investigate the desulfurization of various sulfur-containing heterocyclic compounds in the presence of various organic solvents. P. putida A4 had the same substrate range as R. erythropolis XP and could degrade dibenzothiophene at a specific rate of 1.29 mM g (dry weight) of cells(-1) h(-1) for the first 2 h in the presence of 10% (vol/vol) p-xylene. P. putida A4 was also able to degrade dibenzothiophene in the presence of many other organic solvents at a concentration of 10% (vol/vol). This study is a significant step in the exploration of the biotechnological potential of novel biocatalysts for developing an efficient biodesulfurization process in biphasic reaction mixtures containing toxic organic solvents.