Inducing and Quantifying Clostridium difficile Spore Formation
Inducing and Quantifying Clostridium difficile Spore Formation
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DOI：
10.1007/978-1-4939-6361-4_10
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发表时间：
2016-01-01
期刊：
影响因子：
--
通讯作者：
Pishdadian, Keyan
中科院分区：
文献类型：
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作者：
Shen, Aimee;Fimlaid, Kelly A.;Pishdadian, Keyan
革兰氏阳性医院病原体艰难梭菌在胃肠道中生长期间诱导孢子形成。孢子形成是这种专性厌氧菌形成代谢休眠孢子所必需的，这些孢子可以抵抗抗生素治疗，从哺乳动物宿主中存活下来，并传播C。艰难感染。在本章中，我们描述了一种诱导C的方法。体外艰难孢子形成。该方法可用于研究孢子形成和最大限度地提高孢子纯化产量的一些C。艰难菌株背景。我们还描述了程序可视化孢子形成使用相差显微镜和量化的效率，孢子形成使用耐热性作为衡量功能性孢子形成。
The Gram-positive nosocomial pathogen Clostridium difficile induces sporulation during growth in the gastrointestinal tract. Sporulation is necessary for this obligate anaerobe to form metabolically dormant spores that can resist antibiotic treatment, survive exit from the mammalian host, and transmit C. difficile infections. In this chapter, we describe a method for inducing C. difficile sporulation in vitro. This method can be used to study sporulation and maximize spore purification yields for a number of C. difficile strain backgrounds. We also describe procedures for visualizing spore formation using phase-contrast microscopy and for quantifying the efficiency of sporulation using heat resistance as a measure of functional spore formation.