An integrated strategy for establishment of metabolite profile of endogenous lysoglycerophospholipids by two LC-MS/MS platforms.
An integrated strategy for establishment of metabolite profile of endogenous lysoglycerophospholipids by two LC-MS/MS platforms.
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DOI：
10.1016/j.talanta.2016.10.045
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发表时间：
2017
期刊：
影响因子：
6.1
通讯作者：
Guiying Chen;Chengwu Song;Shuna Jin;Sen Li;Yang Zhang;Rongzeng Huang;Yulin Feng;Yong Xu;
中科院分区：
文献类型：
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作者：
Guiying Chen;Chengwu Song;Shuna Jin;Sen Li;Yang Zhang;Rongzeng Huang;Yulin Feng;Yong Xu;
内源性磷脂（PL）的检测方法主要集中在液相色谱-串联质谱（LC-MS/MS）的应用。由于甘油磷脂的结构关系和相似的核心结构导致了共同的和可预测的质谱裂解行为，在本研究中，采用液相色谱-四极杆线性离子阱质谱（LC-MS）多反应监测（MRM）模式对6种溶血甘油磷脂（Lyso-PL）进行预测-验证在生物血清样品中，使用液相色谱-四极杆飞行时间质谱（LC-QTOF-MS/MS）补充LC-QTRAP-MS/MS。建立了Lyso-PL的靶向代谢物谱，并以LC-QTRAP-MRM分析的形式表示。在人和四种啮齿动物血清中共检测到96种Lyso-PL分子，包括317种区域异构体。此外，该代谢物谱成功地应用于人和四种啮齿动物血清中Lyso-PLs的定性分布分析。我们相信，这种改进的方法，内源性溶血磷脂的定性分析，应大大有助于确定生物系统中的脂质分子种类的作用。此外，建立代谢物谱的综合策略可应用于其他内源性代谢物的靶向定性分析，这些代谢物存在于高个体数量中，但具有结构相似性或相似的官能团。
The methods for detecting endogenous phospholipids (PL) are focused on the use of liquid chromatography-tandem mass spectrometry (LC-MS/MS). Since the structural relation and similar core structure of glycerophospholipids results in a common and predictable mass spectrometric fragmentation behavior, in the present study, six classes of lysoglycerophospholipid (Lyso-PL) were subjected to a predict-verify approach by multiple reaction monitoring (MRM) mode based on liquid chromatography-quadrupole linear ion trap mass spectrometry (LC-QTRAP-MS/MS) complemented with liquid chromatography-quadrupole time of flight mass spectrometry (LC-QTOF-MS/MS) in biological serum samples. A targeted metabolite profile of Lyso-PL was established and represented in the form of a LC-QTRAP-MRM analysis. There were total of 96 Lyso-PL molecular species, consisting of 317 regioisomers detected in serum of human and four rodent species. In addition, the metabolite profile was successfully applied to the analysis of qualitative distribution of Lyso-PLs in serum of human and four rodent species. We believe that this improvement in the method for qualitative analysis of endogenous Lyso-PLs should greatly contribute to identifying the role of lipid molecular species in biological systems. Also, the integrated strategy for establishment of metabolite profile can be applied to targeted qualitative analysis of other endogenous metabolites which occur in a high individual number but sharing either structural similarities or similar functional groups.