Murine B7 antigen provides an efficient costimulatory signal for activation of murine T lymphocytes via the T-cell receptor/CD3 complex.
Murine B7 antigen provides an efficient costimulatory signal for activation of murine T lymphocytes via the T-cell receptor/CD3 complex.
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DOI：
10.1073/pnas.89.1.271
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发表时间：
1992
影响因子：
11.1
通讯作者：
Hans Reiser;G. Freeman;Z. Razi‐Wolf;Claude D. Gimmi;B. Benacerraf;Lee M. Nadler
中科院分区：
文献类型：
--
作者：
Hans Reiser;G. Freeman;Z. Razi‐Wolf;Claude D. Gimmi;B. Benacerraf;Lee M. Nadler
我们证明，小鼠B7（mB 7）蛋白是一种有效的共刺激分子，通过T细胞受体（TCR）/CD 3复合物激活静息小鼠CD 4 + T细胞。稳定的mB 7转染的中国仓鼠卵巢细胞，而不是载体转染的对照，协同抗CD 3单克隆抗体和Con A诱导的T细胞活化，最终导致增殖。mB 7通过诱导白细胞介素2的产生和白细胞介素2受体的表达发挥其作用。因此，mB 7通过正向调节T细胞扩增的正常途径，通过TCR/CD 3复合物共刺激T细胞活化。与mB 7通过TCR/CD 3复合物对T细胞活化的显著作用相反，mB 7转染的CHO细胞系仅低效地通过糖基磷脂酰肌醇锚定蛋白Thy-1和Ly-6A.2共刺激T细胞活化。最后，钙离子载体和mB 7的组合不足以引起T细胞增殖，而钙离子载体和佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）的组合有效地刺激T细胞。因此，mB 7和PMA提供的鼠T淋巴细胞活化信号是不可互换的，尽管两者都通过TCR/CD 3复合物共刺激活化。
We demonstrate that the murine B7 (mB7) protein is a potent costimulatory molecule for the activation of resting murine CD4+ T cells through the T-cell receptor (TCR)/CD3 complex. Stable mB7-transfected Chinese hamster ovary cells, but not vector-transfected controls, synergize with anti-CD3 monoclonal antibody and Con A-induced T-cell activation, resulting ultimately in proliferation. mB7 exerted its effect by inducing production of interleukin 2 and expression of the interleukin 2 receptor. Thus, mB7 costimulates T-cell activation through the TCR/CD3 complex by positively modulating the normal pathway of T-cell expansion. In contrast to the pronounced effect of mB7 on the activation of T cells through the TCR/CD3 complex, the mB7-transfected CHO cell line costimulated T-cell activation via the glycosylphosphatidylinositol-anchored proteins Thy-1 and Ly-6A.2 only inefficiently. Finally, the combination of a calcium ionophore and mB7 is not sufficient to cause T-cell proliferation, while the combination of a calcium ionophore and phorbol 12-myristate 13-acetate (PMA) stimulates T cells efficiently. The signals that mB7 and PMA provide for murine T lymphocyte activation are therefore not interchangeable, although both costimulate activation through the TCR/CD3 complex.