Expression of the heat shock gene hsp16.6 and promoter analysis in the cyanobacterium, Synechocystis sp PCC 6803
Expression of the heat shock gene hsp16.6 and promoter analysis in the cyanobacterium, Synechocystis sp PCC 6803
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DOI：
10.1007/s00284-004-4340-5
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发表时间：
2004-09-01
影响因子：
2.6
通讯作者：
Barnum, SR
中科院分区：
文献类型：
--
作者：
Fang, F;Barnum, SR
对蓝藻Synechocystis sp. PCC 6803的hsp16.6及其上游区进行了分析。hsp16.6转录起始点位于ATG翻译起始密码子上游44个碱基对（bp）处。将265 bp的上游片段连接到lacZ编码序列的上游区域，构建报告载体。β -半乳糖苷酶分析表明，265 bp区域不诱导lacZ基因在大肠杆菌中的表达；尽管使用聚囊藻groESL启动子可诱导表达。在聚囊藻细胞中，265 bp片段作为启动子表达lacZ。冷胁迫和乙醇不诱导lacZ表达，而热休克、盐胁迫、山梨醇、过氧化氢和强光诱导lacZ表达。265 bp区域的一系列缺失表明，-35附近的区域是hsp16.6表达所必需的。
hsp16.6 and its upstream region from the cyanobacterium, Synechocystis sp. PCC 6803, have been analyzed. The hsp16.6 transcriptional start point was positioned 44 base pairs (bp) upstream of the ATG translation start codon. A reporter vector was constructed by ligating the 265 bp upstream fragment onto the upstream region of the lacZ coding sequence. beta-galactosidase analysis indicated that the 265 bp region did not induce lacZ gene expression in E. coli; although expression was induced when the Synechocystis groESL promoter was used. In Synechocystis cells, lacZ was expressed when the 265 bp fragment was used as a promoter. Cold stress and ethanol did not induce lacZ expression, while heat shock, salt stress, sorbitol, hydrogen peroxide, and high light induced lacZ, A series of deletions from the 265 bp region demonstrated that a region around -35 was essential for hsp16.6 expression.