Retinoic Acid Is Sufficient for the In Vitro Induction of Mouse Spermatocytes.
Retinoic Acid Is Sufficient for the In Vitro Induction of Mouse Spermatocytes.
复制标题
视黄酸足以进行小鼠精母细胞的体外诱导。
DOI：
10.1016/j.stemcr.2016.05.013
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发表时间：
2016-07-12
影响因子：
5.9
通讯作者：
Han C
中科院分区：
文献类型：
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作者：
Wang S;Wang X;Ma L;Lin X;Zhang D;Li Z;Wu Y;Zheng C;Feng X;Liao S;Feng Y;Chen J;Hu X;Wang M;Han C
减数分裂是配子发生的关键步骤。然而，由于缺乏体外模型，哺乳动物减数分裂的机制仍然知之甚少。在这里，我们报道视黄酸（RA）足以从培养的小鼠精原干细胞中诱导细线期/合子精母细胞。通过 RNA 测序鉴定出多个受 RA 调控的基因。 RA 与幼犬支持细胞共培养相结合，诱导效率高达 28%。诱导的生精细胞和分离的生精细胞的转录组谱的比较揭示了生殖细胞的渐进诱导。使用该模型，我们表明 Stra8、Agpat3、Fam57a、Wdr91 和 Sox30 对增殖和减数分裂起始的贡献不同。总之，我们使用基于 RA/幼崽支持细胞的体外模型有效地生成了精母细胞，并为其在识别哺乳动物减数分裂相关基因中的应用提供了概念验证证据。视黄酸 (RA) 足以诱导精母细胞 RA 激活减数分裂基因，同时抑制 SSC 自我更新基因 建立了基于 RA 和幼代支持细胞的减数分裂体外模型 使用体外模型已鉴定出减数分裂基因 在本文中，Han 及其同事表明，调节大量基因的视黄酸 (RA) 就足够了 用于从培养的小鼠精原干细胞中诱导精母细胞。幼鼠的 RA 和支持细胞共培养构成了更高效率的体外减数分裂模型。作者进一步表明，该模型可用于识别减数分裂基因。
Meiosis is the key step in gametogenesis. However, the mechanism of mammalian meiosis remains poorly understood due to the lack of an in vitro model. Here, we report that retinoic acid (RA) is sufficient for inducing leptotene/zygotene spermatocytes from cultured mouse spermatogonial stem cells. Multiple genes regulated by RA were identified by RNA sequencing. RA in combination with pup Sertoli cell co-culture resulted in a higher induction efficiency of 28%. Comparisons in the transcriptomic profiles of the induced spermatogenic cells and the isolated ones revealed the progressive induction of the germ cells. Using this model, we showed that Stra8, Agpat3, Fam57a, Wdr91, and Sox30 contributed to the proliferation and meiosis initiation differentially. In conclusion, we have efficiently generated spermatocytes using an RA/pup Sertoli cell-based in vitro model and provided proof-of-concept evidence for its application in identifying genes involved in mammalian meiosis. Retinoic acid (RA) is sufficient for the induction of spermatocytes RA activates meiotic genes while repressing genes for SSC self-renewal An in vitro model for meiosis based on RA and pup Sertoli cells is established Genes for meiosis have been identified using the in vitro model In this article, Han and colleagues show that retinoic acid (RA), which regulates a large number of genes, is sufficient for inducing spermatocytes from cultured mouse spermatogonial stem cells. RA and Sertoli cell co-culture from pup mice constitute an in vitro meiosis model of higher efficiency. The authors further show that this model can be used to identify meiotic genes.