Measurement of retinal blood velocity and flow rate in primates using a liposome-dye system.
Measurement of retinal blood velocity and flow rate in primates using a liposome-dye system.
复制标题
使用脂质体染料系统测量灵长类动物的视网膜血流速度和流速。
DOI：
10.1016/s0161-6420(89)32807-7
复制
发表时间：
1989
期刊：
影响因子：
13.7
通讯作者：
Oncel,M
中科院分区：
文献类型：
--
作者：
Khoobehi,B;Peyman,GA;Niesman,MR;Oncel,M
最近开发的脂质体染料方法用于定量测量恒河猴视网膜中的血流速度和体积流速。全身注射脂质体封装的荧光染料（钙黄绿素）后，由眼科氩激光触发在视网膜中释放一团染料。最初，钙黄绿素以高浓度被捕获，并且脂质囊泡中的染料的荧光被猝灭。激光照射后，脂质体在视网膜特定血管的激光照射部位释放一团染料。释放的染料在暴露的动脉或静脉中形成清晰的荧光波前。通过测量染料在设定时间间隔内在静脉中行进的距离，可以测量不同直径的视网膜血管中的血流速度，并计算这些血管中的体积流速。
A recently developed liposome-dye method was used to make quantitative measurements of blood velocity and volumetric flow rates in the retina of the rhesus monkey. After systemic injection of a liposome-encapsulated fluorescent dye (calcein), the release of a bolus of the dye in the retina was triggered by an ophthalmic argon laser. Initially, calcein was entrapped at a high concentration and the fluorescence of the dye in the lipid vesicle was quenched. After laser exposure, the liposomes released a bolus of dye at the laser exposure site in a specific vessel of the retina. The released dye formed a well-defined, fluorescent wavefront in the exposed artery or vein. By measuring the distance the dye traveled in the vein over a set time interval, it was possible to measure blood velocity in retinal vessels of various diameters, and to calculate volumetric flow rates in those vessels.