Tumor necrosis factor alpha-induced glucose transporter (GLUT-1) mRNA stabilization in 3T3-L1 preadipocytes. Regulation by the adenosine-uridine binding factor.
Tumor necrosis factor alpha-induced glucose transporter (GLUT-1) mRNA stabilization in 3T3-L1 preadipocytes. Regulation by the adenosine-uridine binding factor.
复制标题
3T3-L1 前脂肪细胞中肿瘤坏死因子 α 诱导的葡萄糖转运蛋白 (GLUT-1) mRNA 稳定。
DOI：
10.1016/s0021-9258(18)42448-9
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
J. Malter
中科院分区：
文献类型：
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作者：
J. Stephens;B. Carter;P. Pekala;J. Malter
肿瘤坏死因子α(TNFα)、12-O-十四酰佛波醇-13-乙酸酯和cAMP通过稳定编码基础葡萄糖转运体GLUT-1的相对不稳定的mRNA，刺激静止的3T3-L1前脂肪细胞的己糖转运。GLUT-1mRNA的3‘-UTR包含富AU区背景下破坏稳定的AUUUA基序的单一副本。腺苷尿苷结合因子(AUBF)是一种胞质蛋白，与多种不稳定的细胞因子和癌基因mRNAs中类似的AU富集区相互作用。在这里，我们证明了AUBF复合物在体外通过3‘-UTR的AU富含部分与GLUT-1mRNA结合。在静止的前脂肪细胞中，AUBF活性很低，但可以被TPA、TNFα、cAMP和冈田酸等激动剂上调，所有这些激动剂都能稳定Glut-1mRNA。肿瘤坏死因子α和TPA介导的AUBF上调和GLUT-1mRNA稳定的时间过程是一致的，提示存在因果关系。
Tumor necrosis factor alpha (TNF alpha), 12-O-tetradecanoylphorbol-13-acetate and cAMP stimulate hexose transport in quiescent 3T3-L1 preadipocytes by stabilizing the relatively labile mRNA coding for the basal glucose transporter, GLUT-1. The 3‘-UTR of GLUT-1 mRNA contains a single copy of the destabilizing AUUUA motif in the context of an AU-rich region. The adenosine-uridine binding factor (AUBF) is a cytosolic protein which interacts with similar AU-rich regions in a variety of labile cytokine and oncogene mRNAs. Here, we demonstrate that AUBF complexes in vitro with GLUT-1 mRNA through the AU-rich portion of the 3‘-UTR. AUBF activity is very low in quiescent preadipocytes, but can be up-regulated by agonists such as TPA, TNF alpha, cAMP, and okadaic acid, all of which stabilize GLUT-1 mRNA. The time courses of TNF alpha- and TPA-mediated AUBF up-regulation and GLUT-1 mRNA stabilization are coincident, suggesting a cause and effect relationship.