NUCLEOTIDE-SEQUENCE OF THE SUF2 FRAMESHIFT SUPPRESSOR GENE OF SACCHAROMYCES-CEREVISIAE
NUCLEOTIDE-SEQUENCE OF THE SUF2 FRAMESHIFT SUPPRESSOR GENE OF SACCHAROMYCES-CEREVISIAE
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DOI：
10.1073/pnas.79.11.3565
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
CULBERTSON, MR
中科院分区：
文献类型：
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作者：
CUMMINS, CM;DONAHUE, TF;CULBERTSON, MR
为了阐明酵母SUF 2基因抑制移码的分子机制，测定了携带该基因的SUF 2 -1和suf 2+等位基因的DNA片段及其周围区域的序列。抑制基因和野生型序列的比较表明，SUF 2基因产物是脯氨酸tRNA。不考虑可能的碱基修饰，从DNA序列推断的tRNA的野生型suf 2+反密码子是3“-GGA-5”。SUF 2 -1突变代表G. cntdot的插入。在基因中对应于tRNA的反密码子环的位置处的C碱基对。用3“-GGGA-5”4-碱基反密码子替换野生型suf 2+反密码子使得SUF 2 -1 tRNA能够抑制由于his 4 -712和his 4 -713移码突变而产生的5“-CCCU-3”4-碱基密码子。这种非三联体密码子-反密码子相互作用恢复了正确的阅读框架，并允许合成功能性的his 4蛋白。
To elucidate the molecular mechanism of frameshift suppression by the SUF2 gene of yeast, the sequences of DNA fragments carrying the SUF2-1 and suf2+ alleles of the gene and surrounding regions were determined. Comparison of the suppressor and wild-type sequences indicates that the SUF2 gene product is a proline tRNA. Disregarding possible base modifications, the wild-type suf2+ anticodon of the tRNA inferred from the DNA sequence is 3''-GGA-5''. The SUF2-1 mutation represents the insertion of a G .cntdot. C base pair at a position in the gene that corresponds to the anticodon loop of the tRNA. Replacement of the wild-type suf2+ anticodon by 3''-GGGA-5'' 4-base anticodon enables the SUF2-1 tRNA to suppress the 5''-CCCU-3'' 4-base codons generated as the result of the his4-712 and his4-713 frameshift mutations. This nontriplet codon-anticodon interaction restores the correct reading frame and allows synthesis of a functional his4 protein.