Addressing various compartments of the diatom model organism Phaeodactylum tricornutum via sub-cellular marker proteins
Addressing various compartments of the diatom model organism Phaeodactylum tricornutum via sub-cellular marker proteins
复制标题
DOI：
10.1016/j.algal.2016.10.018
复制
发表时间：
2016-12-01
影响因子：
5.1
通讯作者：
Zauner, Stefan
中科院分区：
文献类型：
--
作者：
Liu, Xiaojuan;Hempel, Franziska;Zauner, Stefan
硅藻三角褐指藻是一种重要的生物体，用于研究蛋白质输入到由四层膜包围的复杂质体中的机制。在这方面，针对这种生物体采用并建立了各种分子技术，因此当今各种研究学科的广泛问题都可以得到解决。至少在亚细胞定位研究的情况下，这种结构复杂的生物体的不同区室必须通过标记分子来定位。在这里，我们展示了一系列亚细胞标记蛋白，它们甚至在亚区室水平上也提供了明确的定位，并暗示了进一步研究的新的、令人着迷的主题。 (C) 2016 Elsevier B.V. 保留所有权利。
The diatom Phaeodactylum tricornutum is a prominent organism to study protein import mechanisms into complex plastids surrounded by four membranes. In this regard, various molecular techniques were adapted and established for this organism, so that nowadays a broad range of questions in a variety of research disciplines can be addressed. At least in the case of sub-cellular localization studies, the different compartments of this complex structured organism have to be addressed by marker molecules. Here we present an arsenal of subcellular marker proteins, which provide defined localizations even on the sub-compartmental level and imply new and fascinating topics for further research. (C) 2016 Elsevier B.V. All rights reserved.