Characterization of sinusoidal endothelial cells of the liver and bone marrow using an intravital lectin injection method
Characterization of sinusoidal endothelial cells of the liver and bone marrow using an intravital lectin injection method
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DOI：
10.1007/s10735-008-9186-x
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发表时间：
2008-10-01
影响因子：
3.2
通讯作者：
Ezaki, Taichi
中科院分区：
文献类型：
--
作者：
Nakamura-Ishizu, Ayako;Morikawa, Shunichi;Ezaki, Taichi
血管内皮细胞表达多种结构和生物学表型。我们使用植物源凝集素（番茄凝集素（LEL）、蓖麻凝集素-I（RCA-I）、欧洲荆豆凝集素-I（UEA-I）和伴刀豆球蛋白A（ConA））的活体注射方法来阐明肝脏和骨髓窦内皮的形态和功能。所有四种凝集素均以斑片状颗粒图案对肝脏和骨髓的肝窦内皮进行染色，这与其他器官中非肝窦血管的均匀且光滑的染色图案不同。通过透射电子显微镜，颗粒图案被鉴定为这些凝集素的内化以及随后在内皮细胞内的积累，表明它们具有活跃的内吞作用。这些凝集素的内吞作用强调了这样一个事实：肝脏和骨髓的窦内皮细胞属于网状内皮系统（RES），这是一种以外来物质内化为特征的细胞系统。我们介绍这种活体凝集素注射作为一种有用的技术来区分肝脏和骨髓的窦内皮与其他血管内皮。
The vascular endothelia express a variety of structural and biological phenotypes. We used an intravital injection method of plant derived lectins (Lycopersicon esculentum lectin (LEL), Ricinus communis Agglutinin-I (RCA-I), Ulex europaeus Agglutinin-I (UEA-I) and Concanavalin A (ConA)) to elucidate the morphology and function of the sinusoidal endothelium of the liver and bone marrow. All four lectins stained the sinusoidal endothelia of the liver and bone marrow in a patchy granular pattern which differed from the uniform and smooth staining pattern of non-sinusoidal vessels in other organs. By transmission electron microscopy, the granular pattern was identified as internalization of these lectins and subsequent accumulation within the endothelial cells, suggesting their active endocytosis. The endocytosis of these lectins emphasizes the fact that sinusoidal endothelial cells of the liver and bone marrow belong to the reticuloendothelial system (RES), a cell system characterized by internalization of foreign material. We introduce this intravital lectin injection as a useful technique to discriminate sinusoidal endothelial of the liver and bone marrow from other vascular endothelia.