Ras-dependent cell cycle commitment during G2 phase.
Ras-dependent cell cycle commitment during G2 phase.
复制标题
G2 期 Ras 依赖性细胞周期定型。
DOI：
10.1016/s0014-5793(01)02115-9
复制
发表时间：
2001
期刊：
影响因子：
3.5
通讯作者：
Stacey,DW
中科院分区：
文献类型：
--
作者：
Hitomi,M;Stacey,DW
Synchronization used to study cell cycle progression may change the characteristics of rapidly proliferating cells. By combining time-lapse, quantitative fluorescent microscopy and microinjection, we have established a method to analyze the cell cycle progression of individual cells without synchronization. This new approach revealed that rapidly growing NIH3T3 cells make a Ras-dependent commitment for completion of the next cell cycle while they are in G2 phase of the preceding cell cycle. Thus, Ras activity during G2 phase induces cyclin D1 expression. This expression continues through the next G1 phase even in the absence of Ras activity, and drives cells into S phase.
