Pharmacokinetics of luteolin and tetra-acetyl-luteolin assayed by HPLC in rats after oral administration
Pharmacokinetics of luteolin and tetra-acetyl-luteolin assayed by HPLC in rats after oral administration
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DOI：
10.1002/bmc.1370
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发表时间：
2010-08-01
影响因子：
1.8
通讯作者：
Bi, Kaishun
中科院分区：
文献类型：
--
作者：
Chen, Xiujie;Liu, Lei;Bi, Kaishun
建立了测定大鼠血浆中木犀草素（LT）和四乙酰木犀草素（TALT）浓度的高效液相色谱法。HPLC分析在由保护Agilent Zorbax Eclipse Plus保护的Agilent TC-C-18柱上进行。LT的移动的流动相为乙腈-水（40：60，v/v）（含0.5%磷酸），流速为1.0 mL/min; TALT的流动相为甲醇-水（70：30，v/v）（含0.5%冰醋酸），流速相同。两种分析物的UV检测波长设定为350 nm。校准曲线在40-1800 ng/mL范围内呈线性，IT和TALT的定量下限为40 ng/mL，检测下限为20 ng/mL。所有样品的日内和日间精密度（RSD）值均在7.9%以内。经口给药（30 mg/kg）后，LT和TALT的浓度-时间曲线均符合二室模型。TALT的最大血药浓度（C-max）和药时曲线下面积（AUC）均优于LT。版权所有（C）2010约翰威利父子有限公司
Accurate and reproducible HPLC methods were developed and validated for the determination of concentrations of luteolin (LT) and tetra-acetyl-luteolin (TALT) in rat plasma. HPLC analyses were performed on an Agilent TC-C-18 column protected by a guard Agilent Zorbax Eclipse Plus. The mobile phase for LT was a binary mixture of acetonitrile-water (40:60, v/v) containing 0.5% phosphoric acid at a flow rate of 1.0 mL/min, and that for TALT was a binary mixture of methanol-water (70:30, v/v) containing 0.5% glacial acetic acid at the same flow rate. The UV detection wavelength for both analytes was set at 350 nm. The calibration curve was linear over the range of 40-1800 ng/mL, the lower limit of quantitation was 40 ng/mL and the lower limit of detection was 20 ng/mL for both IT and TALT. The intra- and inter-day precision (RSD) values for all samples were within 7.9%. The concentration-time curves of LT and TALT after oral administration (30 mg/kg) were both fitted to a two-compartment model. The pharmacokinetic characteristics of TALT were better than that of LT in the maximum plasma concentration (C-max) and the area under the concentration-time curve (AUC). Copyright (C) 2010 John Wiley & Sons, Ltd.