Exposure of lentiviral vectors to subneutral pH shifts the tropism from Purkinje cell to Bergmann glia
Exposure of lentiviral vectors to subneutral pH shifts the tropism from Purkinje cell to Bergmann glia
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DOI：
10.1111/j.1460-9568.2006.04927.x
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发表时间：
2006-07-01
影响因子：
3.4
通讯作者：
Hirai, Hirokazu
中科院分区：
文献类型：
--
作者：
Torashima, Takashi;Yamada, Nobuaki;Hirai, Hirokazu
小脑浦肯野细胞在小脑功能中起重要作用;浦肯野细胞的损伤导致运动协调和运动学习的中断。虽然选择性基因传递到浦肯野细胞将是一个强大的技术在小脑的病理生理学的研究，这样的交付方法尚未建立。在这里，我们采用人类免疫缺陷病毒衍生的慢病毒载体假型与水泡性口炎病毒糖蛋白，以抑制浦肯野细胞，并检查的因素，严重影响病毒的嗜性浦肯野细胞。使用不同的方案产生编码GFP的病毒载体，然后将其注射到小鼠小脑中。在转导后7天和2个月，测定转导的浦肯野细胞的相对比例。从pH 7.2的培养基中收获的慢病毒载体优先转导浦肯野细胞（约一半的转导细胞）。相反，当病毒载体从培养基中收获时，
Cerebellar Purkinje cells play an important role in cerebellar function; lesions of Purkinje cells result in the disruption of motor coordination and motor learning. Although selective gene delivery to Purkinje cells would be a powerful technique for the study of pathophysiology in the cerebellum, a method for such a delivery has not yet been established. Here we employed human immunodeficiency virus-derived lentiviral vectors pseudotyped with vesicular stomatitis virus glycoprotein to transduce Purkinje cells and examined factors that critically affect the viral tropism for Purkinje cells. Viral vectors encoding GFP were generated using different protocols, and were then injected into the mouse cerebellum. At 7 days and 2 months post-transduction, the relative proportions of transduced Purkinje cells were determined. Lentiviral vectors harvested from a medium of pH 7.2 preferentially transduced Purkinje cells (about half of the transduced cells). In contrast, when the viral vector was harvested from medium of