Alkali Metal Cationization of Tumor-associated Antigen Peptides for Improved Dissociation and Measurement by Differential Ion Mobility-Mass Spectrometry.
Alkali Metal Cationization of Tumor-associated Antigen Peptides for Improved Dissociation and Measurement by Differential Ion Mobility-Mass Spectrometry.
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DOI：
10.1021/acs.jproteome.0c00157
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发表时间：
2020-08-07
影响因子：
4.4
通讯作者：
Glish, Gary L.
中科院分区：
文献类型：
--
作者：
Keating, James E.;Chung, Chris;Chai, Shengjie;Prins, Jans F.;Vincent, Benjamin G.;Hunsucker, Sally A.;Armistead, Paul M.;Glish, Gary L.
串联质谱法（MS/MS）是一种高灵敏度和高选择性的检测肿瘤相关肽抗原的方法。这些短的非胰蛋白酶序列可能缺乏碱性残基，导致在电喷雾中主要形成[肽+H]+离子。这些单电荷离子倾向于经历低效解离，导致序列测定中的问题。向电喷雾溶剂中添加碱金属盐可以驱动相对于[肽+H]+离子具有增强的解离特性的[肽+H+金属]2+离子的形成。研究了之前鉴定的肿瘤相关抗原和预测的新抗原序列。先前报道的钠阳离子化肽在MS/MS中的重排机制在这里被应用于证明肿瘤相关肽抗原的完整C-末端测序。示出差分离子迁移谱法（DIMS）选择性地丰富[肽+H+金属]2+物种通过过滤出单电荷的干扰，在相对较低的场强，抵消信号强度的下降与使用碱金属阳离子。
Tandem mass spectrometry (MS/MS) is a highly sensitive and selective method for detection of tumor associated peptide antigens. These short, non-tryptic sequences may lack basic residues, resulting in formation of predominantly [peptide+H]+ ions in electrospray. These singly charged ions tend to undergo inefficient dissociation, leading to issues in sequence determination. Addition of alkali metal salts to the electrospray solvent can drive formation of [peptide+H+metal]2+ ions that have enhanced dissociation characteristics relative to [peptide+H]+ ions. Both previously identified tumor-associated antigens and predicted neoantigen sequences were investigated. The previously reported rearrangement mechanism in MS/MS of sodium cationized peptides is applied here to demonstrate complete C-terminal sequencing of tumor associated peptide antigens. Differential ion mobility spectrometry (DIMS) is shown to selectively enrich [peptide+H+metal]2+ species by filtering out singly charged interferences at relatively low field strengths, offsetting the decrease in signal intensity associated with the use of alkali metal cations.
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影响因子：
32.4
作者：
Abelin JG;Keskin DB;Sarkizova S;Hartigan CR;Zhang W;Sidney J;Stevens J;Lane W;Zhang GL;Eisenhaure TM;Clauser KR;Hacohen N;Rooney MS;Carr SA;Wu CJ
通讯作者：
Wu CJ
影响因子：
1.8
作者：
Bensadek, Dalila;Monigatti, Flavio;Steen, Hanno
通讯作者：
Steen, Hanno
影响因子：
20.3
作者：
Dossa, Robson G.;Cunningham, Tanya;Bleakley, Marie
通讯作者：
Bleakley, Marie
影响因子：
15
作者：
GRESE, RP;GROSS, ML
通讯作者：
GROSS, ML
影响因子：
3.3
作者：
Grewal, RN;El Aribi, H;Hopkinson, AC
通讯作者：
Hopkinson, AC