Behavioral differences in three Wistar Han rat lines for emotional reactivity, cognitive processing and ethanol intake
Behavioral differences in three Wistar Han rat lines for emotional reactivity, cognitive processing and ethanol intake
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DOI：
10.1016/j.physbeh.2012.12.019
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发表时间：
2013-02-17
影响因子：
2.9
通讯作者：
Schneider, Miriam
中科院分区：
文献类型：
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作者：
Goepfrich, Anja A.;Gluch, Christian;Schneider, Miriam
许多实验室从商业供应商处获得实验动物，因此取决于它们的条件和繁殖计划。供应商停止繁殖或更换特定的大鼠品系迫使客户放弃传统的测试动物，并用新的大鼠品系重新建立所有行为范例。因此，了解同一大鼠品系的不同繁殖系中是否出现行为差异至关重要。在最近的一个案例中，商业供应商 Harlan Laboratories GmbH 正在用源自不同种畜的 RccHan:WIST 品系替代之前的 Wistar 大鼠 HsdHan:WIST 品系。因此，我们在广泛的行为范式中测试了这两个品系的动物（来自 Harlan Laboratories GmbH 的 RccHan:WIST 和 HsdHan:WIST）以及同一品系但从不同供应商 (Janvier) 获得的 Wistar 大鼠。我们观察到运动活动、经典焦虑相关范式（高架十字迷宫和光/暗出现测试）以及物体识别记忆和声惊吓反射（ASR）的前脉冲抑制（PPI）方面的差异。我们还发现乙醇摄入量和偏好存在差异，但与美味食物奖励和苦味溶液（奎宁）的摄入量无关。这些结果表明，同一 Wistar 大鼠品系的不同育种品系之间的行为表型存在相当大的差异，旨在提高行为科学家对影响动物行为的品系和供应商差异的认识。 (C) 2013 Elsevier Inc. 保留所有权利。
Many laboratories obtain their experimental animals from commercial suppliers and are therefore dependent on their conditions and breeding schedules. A breeding stop or the substitution of a particular rat line by the supplier forces the customers to abandon their conventional test animals and to re-establish all behavioral paradigms with a new rat line. Therefore, it is vital to know whether behavioral differences emerge in various breeding lines of the same rat strain. In a recent case, the commercial supplier Harlan Laboratories GmbH is substituting the previous HsdHan:WIST line of Wistar rats with the RccHan:WIST line descending from a different breeding stock. We therefore tested animals of both lines (RccHan:WIST and HsdHan:WIST from Harlan Laboratories GmbH) as well as Wistar rats of the same line but obtained from a different supplier (Janvier) in a broad range of behavioral paradigms. We observed differences in locomotor activity, in classical anxiety-related paradigms (elevated plus maze and light/dark emergence test), as well as in object recognition memory and prepulse inhibition (PPI) of the acoustic startle reflex (ASR). We also found differences in ethanol intake and preference, but not regarding the intake of a palatable food reward and a bitter solution (quinine). These results demonstrate considerable variations in the behavioral phenotype between different breeding lines of the same Wistar rat strain and aim to increase the awareness of behavioral scientists for line and supplier differences affecting animal behavior. (C) 2013 Elsevier Inc. All rights reserved.