A novel gradient and multilayered sheet with a silk fibroin/polyvinyl alcohol core?shell structure for bioabsorbable arterial grafts
A novel gradient and multilayered sheet with a silk fibroin/polyvinyl alcohol core?shell structure for bioabsorbable arterial grafts
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一种新型梯度多层片材，具有丝素蛋白/聚乙烯醇核壳结构，用于生物可吸收动脉移植物
DOI：
10.1002/jbm.a.37309
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发表时间：
2021
影响因子：
4.9
通讯作者：
Nakazawa Yasumoto
中科院分区：
文献类型：
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作者：
Koyanagi Eri;Tara Shuhei;Sakata Chiemi;Shimada Kazumi;Kato Konosuke;Miyachi Hideki;Tanaka Ryou;Nakazawa Yasumoto
生物可吸收的动脉移植物可以潜在地改善血管通畅和新血管的形成；然而，它们在临床环境中的应用尚未实现。在这项研究中，我们开发了基于丝素（SF）和聚乙烯醇（PVA）的具有核-壳纳米纤维结构的生物可吸收梯度片。在保持其物理特性和从管腔表面逐渐降解过程的同时，这种梯度片有望促进血管重塑。在不同的流量下对SF和PVA进行ESP，以实现多层梯度结构。增加PVA的流量可以提高梯度板的弹性；然而，这降低了核-壳纤维的抗拉强度。值得注意的是，即使在磷酸盐缓冲盐水溶液中浸泡7天后，梯度片的物理性能也没有下降，这表明该结构在抵抗动脉压的同时仍能保持其结构完整性。体外实验表明，SF/PVA片上的内皮细胞数量明显高于细胞培养皿。将梯度片植入大鼠腹主动脉，并在14天后移植，以证实急性期血管通畅和血管重构。由聚氨酯和聚乙烯醇组成的SF梯度片材改善了材料处理的便利性，这些片材产生了良好的血管重塑效果。我们的研究结果强烈表明，本研究中描述的基于SF/PVA的梯度片在进一步修改后可以作为生物可吸收动脉移植物的新设计。
Bioabsorbable arterial grafts can potentially improve patency and neovessel formation; however, their application in clinical settings has not been realized. In this study, we developed bioabsorbable gradient sheets based on silk fibroin (SF) and polyvinyl alcohol (PVA) with a core–shell nanofibrous structure. This gradient sheet was expected to promote vascular remodeling while we maintained its physical properties and a gradual degrading process from the luminal surface. ESP was conducted at various flow rates for SF and PVA to achieve the multilayer gradient structure. Furthermore, the elasticity of the gradient sheet could be increased by increasing the PVA flow rate; however, this reduced the tensile strength of the core–shell fibers. Notably, the physical properties of the gradient sheet did not degrade even after 7 days of immersion in a phosphate buffer saline solution, which indicates that the structure could maintain its structural integrity while resisting arterial pressure. In vitro experiments revealed that the number of endothelial cells attached to the SF/PVA sheet was notably higher than that on the cell‐culture dish. The gradient sheets were implanted in rat abdominal aortas and explanted after 14 days to confirm acute‐phase patency and vascular remodeling. The gradient sheets constructed with SF composed of polyurethane and PVA improved the ease of handling of the material, and these sheets resulted in a favorable vascular remodeling outcome. Our results strongly suggest that the SF/PVA‐based gradient sheets described in this study can serve as a novel design for bioabsorbable arterial grafts upon further modifications.