Microfibril-associated glycoprotein-1 and fibrillin-2 are associated with tropoelastin deposition in vitro
Microfibril-associated glycoprotein-1 and fibrillin-2 are associated with tropoelastin deposition in vitro
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DOI：
10.1016/j.biocel.2004.06.002
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发表时间：
2005-01-01
影响因子：
4
通讯作者：
Irie, K
中科院分区：
文献类型：
--
作者：
Tsuruga, E;Yajima, T;Irie, K
弹性系统纤维由微原纤维和对弹力蛋白组成。在发育过程中，微原纤维作为模板沉积对流层弹性蛋白。微原纤维相关糖蛋白-1 （MAGP-1）和纤原蛋白-2是微原纤维的主要成分，它们为弹力蛋白沉积提供了可能的模板。在这项研究中，我们使用RNA干扰（RNAi）技术在弹性蛋白产生细胞（人牙龈成纤维细胞）中分别建立了MAGP-1和fibrin -2基因特异性敲低。然后我们用免疫印迹法检测细胞外弹力蛋白的沉积。这两个基因被特异性抑制
Elastic system fibers consist of microfibrils and tropoelastin. During development, microfibrils act as a template on which tropoelastin is deposited. Microfibril-associated glycoprotein-1 (MAGP-1) and fibrillin-2, the major components of microfibrils, provide the likely template for tropoelastin deposition. In this study, we used the RNA interference (RNAi) technique to establish MAGP-1 and fibrillin-2 gene-specific knock-downs individually in elastin-producing cells (human gingival fibroblasts). We then examined the extracellular deposition of tropoelastin by western blotting. These two genes were specifically suppressed to