DELTA-9-TETRAHYDROCANNABINOL ENHANCES RELEASE OF HERPES-SIMPLEX VIRUS TYPE-2
DELTA-9-TETRAHYDROCANNABINOL ENHANCES RELEASE OF HERPES-SIMPLEX VIRUS TYPE-2
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DOI：
10.1099/0022-1317-67-9-2017
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发表时间：
1986-09-01
影响因子：
3.8
通讯作者：
MISHKIN, EM
中科院分区：
文献类型：
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作者：
CABRAL, GA;MCNERNEY, PJ;MISHKIN, EM
本研究旨在探讨微摩尔浓度的增量-9-四氢大麻酚(Delta-9-THC)对单纯疱疹病毒2型(HSV-2)体外复制的影响。病毒感染的Vero细胞经10-5M-或10-6M-Delta-9-THC处理24 h后，感染性胞外病毒数增加100倍。经药物处理的细胞的透射电子显微镜显示质膜溶解，光滑和粗面内质网扩张，并在含有病毒聚集体的细胞质中出现大空泡。这些结果表明，Delta-9-THC通过干扰病毒感染细胞的细胞膜，促进HSV-2的释放。
This study was undertaken to determine the effect of micromolar concentrations of delta-9-tetrahydrocannabinol (Delta-9-THC) on herpes simplex virus type 2 (HSV-2) replication in vitro. Virus-infected Vero cells pretreated for 24 h with 10-5 M- or 10-6 M-Delta-9-THC yielded 100-fold increases in infectious extracellular virus. Transmissionelectron microscopy of drug-treated cells revealed plasma membrane dissolution, distension of the smooth and rough endoplasmic reticulum, and the appearance of macrovacuoles in the cytoplasm containing aggregates of virus. These results suggest that Delta-9-THC enhances the release of HSV-2 by perturbing cellular membranes in virus-infected cells.