Role of Vertical Transmission of Shoot Endophytes in Root-Associated Microbiome Assembly and Heavy Metal Hyperaccumulation in Sedum alfredii
Role of Vertical Transmission of Shoot Endophytes in Root-Associated Microbiome Assembly and Heavy Metal Hyperaccumulation in Sedum alfredii
复制标题
芽内生菌垂直传播在根部相关微生物组组装和景天重金属超积累中的作用
DOI：
10.1021/acs.est.9b01093
复制
发表时间：
2019-06-18
影响因子：
11.4
通讯作者：
Li, Tingqiang
中科院分区：
文献类型：
--
作者：
Luo, Jipeng;Tao, Qi;Li, Tingqiang
根际内生菌的传播方式及其在根微生物组组装和重金属积累中的作用尚不清楚。利用16 S rRNA基因分析技术，研究了东南景天地上部内生菌的垂直传递与生长和Cd/Zn积累的关系。内生菌从嫩枝扦插传播到新植物的根区室，在灭菌（γ射线照射）和原生土壤。垂直传播在无菌土壤中更有效，并且传播的内生菌已成为新建立的根相关微生物组的主要组成部分。根据16 S rRNA基因，垂直传播的分类群被鉴定为链霉菌科、诺卡氏菌科、假诺卡氏菌科和根瘤菌科。链霉菌科，诺卡氏菌科，和假诺卡氏菌科的丰度与增加地上部生物量和总镉/锌积累。接种S. alfredii与垂直传播的内生菌共享相同的系统发育相关性的合成细菌群落导致植物生物量，根系形态和Cd/Zn积累的显着改善。我们的研究结果表明，成功的垂直传播内生菌从芽S。特别是在微生物群落减少的土壤中。此外，内生菌来源的微生物组在金属超积累中起着重要作用。
The transmission mode of shoot-associated endophytes in hyperaccumulators and their roles in root microbiome assembly and heavy metal accumulation remain unclear. Using 16S rRNA gene profiling, we investigated the vertical transmission of shoot-associated endophytes in relation to growth and Cd/Zn accumulation of Sedum alfredii (Crassulaceae). Endophytes were transmitted from shoot cuttings to the rhizocompartment of new plants in both sterilized (gamma-irradiated) and native soils. Vertical transmission was far more efficient in the sterile soil, and the transmitted endophytes have become a dominant component of the newly established root-associated microbiome. Based on 16S rRNA genes, the vertically transmitted taxa were identified as the families of Streptomycetaceae, Nocardioidaceae, Pseudonocardiaceae, and Rhizobiaceae. Abundances of Streptomycetaceae, Nocardioidaceae, and Pseudonocardiaceae were strongly correlated with increased shoot biomass and total Cd/Zn accumulation. Inoculation of S. alfredii with the synthetic bacterial community sharing the same phylogenetic relatedness with the vertically transmitted endophytes resulted in significant improvements in plant biomass, root morphology, and Cd/Zn accumulation. Our results demonstrate that successful vertical transmission of endophytes from shoots of S. alfredii to its rhizocompartments is possible, particularly in soils with attenuated microbiomes. Furthermore, the endophyte-derived microbiome plays an important role in metal hyperaccumulation.