Genotyping of Serratia marcescens Strains Associated with Cucurbit Yellow Vine Disease by Repetitive Elements-Based Polymerase Chain Reaction and DNA-DNA Hybridization.
Genotyping of Serratia marcescens Strains Associated with Cucurbit Yellow Vine Disease by Repetitive Elements-Based Polymerase Chain Reaction and DNA-DNA Hybridization.
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通过基于重复元件的聚合酶链反应和 DNA-DNA 杂交对与葫芦黄藤病相关的粘质沙雷氏菌菌株进行基因分型。
DOI：
10.1094/phyto.2003.93.10.1240
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发表时间：
2003
期刊：
影响因子：
3.2
通讯作者：
J. Fletcher
中科院分区：
文献类型：
--
作者：
Q. Zhang;R. Weyant;A. Steigerwalt;L. White;U. Melcher;B. Bruton;S. Pair;F. Mitchell;J. Fletcher
摘要根据16 SrDNA和groE序列分析，将引起葫芦科植物黄藤病（CYVD）的细菌归为粘质沙雷氏菌（Serratia marcescens）。然而，与S.从各种生态位分离的粘质虫菌株表现出显著的异质性。在本研究中，我们比较了S。不同生态位的粘质沙雷氏菌菌株以及其他沙雷氏菌属的模式菌株。通过基于重复元件的聚合酶链反应（rep-PCR）和DNA-DNA杂交。对于前者，CYVD菌株显示出相同的带型，尽管它们来自不同的葫芦宿主、地理位置和隔离年限。在rep-PCR分析的系统发育树中，CYVD菌株与其他菌株明显不同，但与S.来自其他生态位的粘质虫菌株。DNA相关性研究进一步支持了CYVD菌株的同质性，因为来自哈密瓜分离物C 01-A的标记DNA显示平均相对结合率（RBR）为99%，与其他CYVD菌株的差异为0.33%。C 01-A作为CYVD的代表菌株，其RBR为76%，与S. marcescens型菌株。这些数据证实了先前在S. marcescens。我们的研究，包括rep-PCR，DNA-DNA杂交，和以前的表型实验，已经证明，CYVD相关的S。marcescens群集在一个组中，与该物种的其他菌株显著不同。
ABSTRACT The bacterium that causes cucurbit yellow vine disease (CYVD) has been placed in the species Serratia marcescens based on 16S rDNA and groE sequence analysis. However, phenotypic comparison of the organism with S. marcescens strains isolated from a variety of ecological niches showed significant heterogeneity. In this study, we compared the genomic DNA of S. marcescens strains from different niches as well as type strains of other Serratia spp. through repetitive elements-based polymerase chain reaction (rep-PCR) and DNA-DNA hybridization. With the former, CYVD strains showed identical banding patterns despite the fact that they were from different cucurbit hosts, geographic locations, and years of isolation. In the phylogenetic trees generated from rep-PCR banding patterns, CYVD strains clearly were differentiated from other strains but formed a loosely related group with S. marcescens strains from other niches. The homogeneity of CYVD strains was supported further by the DNA relatedness study, in that labeled DNA from the cantaloupe isolate, C01-A, showed an average relative binding ratio (RBR) of 99%, and 0.33% divergence to other CYVD strains. Used as a representative strain of CYVD, the labeled C01-A had a RBR of 76%, and a 4.5% divergence to the S. marcescens type strain. These data confirm the previous placement of CYVD strains in S. marcescens. Our investigations, including rep-PCR, DNA-DNA hybridization, and previous phenotyping experiments, have demonstrated that CYVD-associated strains of S. marcescens cluster together in a group significantly different from other strains of the species.