Clonal analysis of Hemophilus influenzae isolated from children from Pakistan with lower respiratory tract infections.
Clonal analysis of Hemophilus influenzae isolated from children from Pakistan with lower respiratory tract infections.
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从巴基斯坦患有下呼吸道感染的儿童中分离出的流感嗜血杆菌的克隆分析。
DOI：
10.1093/infdis/160.4.634
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Selander,RK
中科院分区：
文献类型：
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作者：
Weinberg,GA;Ghafoor,A;Ishaq,Z;Nomani,NK;Kabeer,M;Anwar,F;Burney,MI;Qureshi,AW;Musser,JM;Selander,RK
对巴基斯坦下呼吸道感染儿童血液中105株嗜血杆菌分离株的克隆多样性进行分析。十个分离株被鉴定为H。副流感病毒和 95 asH。流感杆菌。流感分离株，61 株（64%）为血清型带，34 株（36%）为不可分型； 95% 的 b 型分离株属于单克隆组（根据多位点酶电泳、SDS-PAGE 外膜蛋白谱和生物型定义）。在美国或欧洲，从患有侵袭性 b 型疾病的患者中恢复的 b 型菌株中很少观察到该克隆。伊斯兰堡的非分型分离株也受到克隆限制：34 个分离株中发现 9 个克隆群，其中仅 5 个克隆群占大多数（82%）菌株。感染 b 型病毒株的儿童比感染非 typepable H 病毒株的儿童住院的频率更高。流感疾病（64% vs. 41%，P= .06），但没有其他临床或人口特征可以区分感染型带不可分型菌株的儿童。
The clonal diversity of 105Hemophilusisolates from the blood of children with lower respiratory tract infection in Pakistan was analyzed. Ten isolates were identified asH. parainfluenzaeand 95 asH. influenzae.Of theH. influenzaeisolates, 61 (64%) were serotype band 34 (36%) were nontypable; 95% of the type b isolates were members of a single clonal group (as defined by multilocus enzyme electrophoresis, SDS-PAGE outer membrane protein profile, and biotype). This clone is rarely observed among type b strains recovered from patients with invasive type b disease in the USA or Europe. The nontypable isolates in Islamabad also were clonally restricted: 9 clonal groups were found among 34 isolates, with just 5 clonal groups accounting for most (82%) of the strains. Children infected with type b strains were hospitalized more often than those with nontypableH. influenzaedisease (64% vs. 41%,P= .06), but no other clinical or demographic features distinguished children infected by type band nontypable strains.