Boronic acid functionalized Fe3 O4 magnetic microspheres for the specific enrichment of glycoproteins.
Boronic acid functionalized Fe3 O4 magnetic microspheres for the specific enrichment of glycoproteins.
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DOI：
10.1002/jssc.201500921
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发表时间：
2016-05
影响因子：
3.1
通讯作者：
Jing Zhang;T. He;L. Tang;Zhiqi Zhang
中科院分区：
文献类型：
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作者：
Jing Zhang;T. He;L. Tang;Zhiqi Zhang
糖蛋白是许多疾病（包括感染和癌症）的有用的生物标志物和治疗靶标。然而，低丰度糖蛋白的鉴定和分离仍然是一个重大挑战，限制了其应用。因此，需要特异性和选择性糖蛋白富集的方法。本研究成功地合成了新型苯硼酸功能化磁性微球。采用水热法合成了Fe 3 O 4微球，并利用超声波法将其包覆在正硅酸乙酯表面形成核壳结构。与常规机械搅拌12 h相比，超声法制备磁性微球的时间缩短约7 h，且自制磁性微球的分散性和均匀性更好。随后，通过依次添加氨基和羧基对磁性微球进行修饰。最后，3-氨基苯硼酸，作为功能单体，连接到磁性微球用于捕获糖蛋白/糖肽。本研究结果表明，苯硼酸功能化磁性微球对糖蛋白/糖肽具有良好的吸附性能。该微球对胎球蛋白的最大吸附量为108 mg/g，富集效率达89.7%，具有分离富集复杂生物样品中糖蛋白的潜力。
Glycoproteins are useful biomarkers and therapeutic targets for a number of diseases, including infections and cancer. However, identification and isolation of low-abundant glycoproteins remains a significant challenge that limits their application. Thus, methods of specific and selective glycoprotein enrichment are required. In this study, novel phenylboronic acid functionalized magnetic microspheres were successfully synthesized. Fe3 O4 microspheres were synthesized by using a hydrothermal method and were coated with tetraethyl orthosilicate using an ultrasonic method to form a core-shell structure. Compared to the conventional mechanical stirring for 12 h, the ultrasonic method saved about 7 h in processing time, and the home-made magnetic microspheres had better dispersibility and homogeneity. Subsequently, the magnetic microspheres were modified by addition of an amino group and a carboxyl group, in sequence. Finally, 3-aminophenylboronic acid, as the functional monomer, was linked to the magnetic microspheres for capturing glycoprotein/glycopeptides. The results of this study indicate that phenylboronic acid functionalized magnetic microspheres show excellent adsorption performance toward glycoprotein/glycopeptides. The maximum absorbing capacity of the microspheres for fetuin was 108 mg/g, and the enrichment efficiency reached 89.7%, indicating their potential to separate and enrich glycoproteins from the complex biological samples.