Development and Characterization of Bilayer Wound Healing Patch Nanofiber Fabricated by Electrospinning
Development and Characterization of Bilayer Wound Healing Patch Nanofiber Fabricated by Electrospinning
复制标题
静电纺丝双层伤口愈合贴片纳米纤维的开发和表征
DOI：
10.4028/www.scientific.net/jnanor.59.46
复制
发表时间：
2019
影响因子：
1.7
通讯作者：
T. Sangnim
中科院分区：
文献类型：
--
作者：
K. Huanbutta;Wancheng Sittikijyothin;T. Sangnim
本研究旨在研制用于伤口敷料的电纺双层聚合物纳米纤维贴片(PNP)。以不同浓度的聚乙烯醇(PVA)和改性罗望子胶负载盐酸克林霉素(CM)为第一层，以Eudradit®S100为第二层，制备了纳米纤维。从扫描电子显微镜的观察结果可以看出，PVA、PVA与树胶的混合物、EUDRAGIT®S100的喷雾产物均为直径在153~1830 nm之间的圆形纤维。聚合物浓度、溶液电导率和表面张力对纳米纤维的外观有影响。在优化的电纺工艺条件下，当外加电压为20kV，注入距离为20 cm，溶液进料速度为0.25mL，集电极滚压速度为48rpm时，成功地制备了纳米纤维双层焊接贴片。差示扫描量热仪(DSC)和X射线衍射仪(PXRD)表明，PNPs中的药物为无定形。生物试验表明，PVA含量为10%、改性罗望子胶含量为5%、克林霉素含量为1%的双层PNPs对金黄色葡萄球菌有较好的抑制作用。这些结果表明，在纳米纤维贴片配方中，使用Eudradit®S100和改性罗望子胶作为天然材料，可以提高纳米纤维贴片的强度。
The present study aims to develop bilayer polymeric nanofiber patch (PNP) fabricated by electrospinning technique using for wound dressing. The nanofiber was prepared by various concentrations of polyvinyl alcohol (PVA) and modified tamarind seed gum loaded with clindamycin HCl (CM) in first layer and Eudragit® S100 for a second layer. According to the SEM result, the physical appearance, the sprayed products prepared from PVA, mixture of PVA and gum, Eudragit® S100 were in round fiber with different diameter size ranged from 153-1830 nm. The polymer concentration, solution conductivity and surface tension affected on the appearance of the nanofiber. The patch was successfully prepared in form of two layer welded with the nanofiber under the optimized electrospinning condition which are 20 kV of applied voltage, 20 cm of injection distance, 0.25 mL of solution feed rate and 48 rpm of collector rolling rate. The DSC and PXRD indicated that the drug in PNPs was in amorphous form. Biological test revealed that bilayer PNPs contained PVA 10%, modified tamarind seed gum 5% and clindamycin 1% had an efficiency to inhibit Staphylococcus aureus. These results show the possibility of improving nanofiber patch strength by using Eudragit® S100 and modified tamarind gum seed as a natural material in nanofiber patch formulation.