Clonostachin, a novel peptaibol that inhibits platelet aggregation
Clonostachin, a novel peptaibol that inhibits platelet aggregation
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DOI：
10.7164/antibiotics.50.105
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发表时间：
1997-02-01
影响因子：
3.3
通讯作者：
Endo, A
中科院分区：
文献类型：
--
作者：
Chikanishi, T;Hasumi, K;Endo, A
从 Clonostachys sp. 培养物中分离出一种新型 peptaibol，命名为 Clonostachin。 F5898经HP-20和硅胶柱色谱及反相HPLC测定。通过 Edman 和化学降解、正离子 FAB-MS、EI-MS 和 NMR 分析确定了克罗穗素的结构。 Clonostachin 是一种线性十四肽，具有 N 末端乙酰基和 C 末端糖醇。 Clonostachin 在 150 muM 时可抑制 ADP 诱导的人血小板聚集 80%。
A novel peptaibol, designated clonostachin, was isolated from cultures of Clonostachys sp. F5898 by HP-20 and silica gel column chromatographies and reverse-phase HPLC. The structure of clonostachin was determined by Edman and chemical degradations, positive ion FAB-MS, EI-MS, and NMR analyses. Clonostachin was a linear tetradecapeptide with an N-terminal acetyl group and a C-terminal sugar alcohol. Clonostachin inhibited ADP-induced aggregation of human platelets by 80% at 150 mu M.