Silicone oil promotes amyloid-like aggregation of alpha B-crystallin
Silicone oil promotes amyloid-like aggregation of alpha B-crystallin
复制标题
硅油促进αB-晶状体蛋白的淀粉样蛋白样聚集
DOI：
10.1039/c6ra27128f
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发表时间：
2017
期刊：
影响因子：
3.9
通讯作者：
Zhang Yi
中科院分区：
文献类型：
--
作者：
Shen Zhiwei;Du Qiqige;Lei Haozhi;Wei Yuhui;Hu Jun;Zhang Yi
硅油是一种化学惰性和生物相容性材料。然而，在视网膜脱离的治疗中，使用硅油作为内填充物的辅助物，伴随着白内障的后遗症，其分子机制一直是科学家们的一个谜。本研究主要研究硅油对眼内重要蛋白α B-晶状体蛋白（αB-crystallin，α AB）聚集行为的影响。我们发现，硅油可以促进淀粉样蛋白聚集的BRIAB，使用原子力显微镜（AFM），透射电子显微镜（TEM），激光粒度和数量分析（Nanosight），和浊度测量验证。此外，使用硫磺素T（ThT）、刚果红和1-苯胺基萘-8-磺酸（ANS）的荧光实验表明，在硅油存在下，在CRAAB中形成β-折叠结构，这也通过远紫外圆二色性（CD）光谱得到证实。这些结果为硅油对眼内淀粉样蛋白聚集行为的影响和ARAAB蛋白二级结构的改变提供了直接证据，有助于理解硅油眼内白内障形成的分子机制。
Silicone oil is a chemically inert and biocompatible material. However, the use of silicone oil as an adjunct for internal tamponade in the treatment of retinal detachment is accompanied by the sequelae of cataract, the molecular mechanism of which has been a mystery for scientists. In this study, we focused on the influence of silicone oil on the aggregating behaviors of one of the important proteins in the eyes, αB-crystallin (CRYAB). We found that silicone oil could promote the amyloid-like aggregation of CRYAB, verified using atomic force microscopy (AFM), transmission electron microscopy (TEM), laser granularity and number analysis (Nanosight), and turbidity measurements. Furthermore, fluorescent experiments using Thioflavin T (ThT), Congo red, and 1-anilinonaphthalene-8-sulfonic acid (ANS) suggested formation of a β-sheet structure in CRYAB in the presence of silicone oil, which was also confirmed by far-UV circular dichroism (CD) spectroscopy. These findings provide a direct evidence of the changes in the secondary structures of CRYAB protein and amyloid aggregation behavior upon adding silicone oil in the solution, and could be helpful for understanding the molecular mechanisms of the cataract formed in silicone oil-filled eyes.