Phosphatidylglycerol is essential for the development of thylakoid membranes in Arabidopsis thaliana
Phosphatidylglycerol is essential for the development of thylakoid membranes in Arabidopsis thaliana
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DOI：
10.1093/pcp/pcf185
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发表时间：
2002-12-01
影响因子：
4.9
通讯作者：
Wada, H
中科院分区：
文献类型：
--
作者：
Hagio, M;Sakurai, I;Wada, H
磷脂酰甘油是许多生物体的生物膜中普遍存在的磷脂。在植物中，磷脂酰甘油主要存在于类囊体膜中，并被认为在光合作用中发挥特定的作用。在这里，我们已经分离出两个T-DNA标记的拟南芥品系，其在编码参与磷脂酰甘油生物合成的磷脂酰甘油磷酸合酶的PGP 1基因中具有T-DNA插入。在T-DNA标记株系的纯合植物中，PGP 1基因被完全破坏。这些敲除突变体的生长依赖于生长培养基中蔗糖的存在，并且这些植物具有浅黄绿色的叶子。由于叶肉细胞数量的减少，突变体的叶片具有非常大的细胞间隙。在突变体中，叶细胞中叶绿体的发育严重受阻。叶肉细胞和叶绿体颗粒只在维管结构周围发现，而表皮细胞扩大，但大部分保存。突变体中磷脂酰甘油的含量降低到野生型的12%。这些结果表明，PGP 1在叶绿体中磷脂酰甘油的生物合成中起着重要作用，磷脂酰甘油对A. thaliana.
Phosphatidylglycerol is a ubiquitous phospholipid in the biological membranes of many organisms. In plants, phosphatidylglycerol is mainly present in thylakoid membranes and has been suggested to play specific roles in photosynthesis. Here, we have isolated two T-DNA tagged lines of Arabidopsis thaliana that have a T-DNA insertion in the PGP1 gene encoding a phosphatidylglycerolphosphate synthase involved in the biosynthesis of phosphatidylglycerol. In homozygous plants of the T-DNA tagged lines, the PGP1 gene was completely disrupted. The growth of these knockout mutants was dependent on the presence of sucrose in the growth medium, and these plants had pale yellow-green leaves. The leaves of the mutants had remarkably large intercellular spaces due to the reduction in the number of mesophyll cells. The development of chloroplasts in the leaf cells was severely arrested in the mutants. Mesophyll cells with chloroplast particles are only found around vascular structures, whereas epidermal cells are enlarged but largely conserved. The content of phosphatidylglycerol in the mutants was reduced to 12% of that of the wild type. These results demonstrate that PGP1 plays a major role in the biosynthesis of phosphatidylglycerol in chloroplasts, and that phosphatidylglycerol is essential for the development of thylakoid membranes in A. thaliana.