Genome-wide survey of transcriptional initiation in the pathogenic fungus, Candida glabrata
Genome-wide survey of transcriptional initiation in the pathogenic fungus, Candida glabrata
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DOI：
10.1111/gtc.12147
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发表时间：
2014-06-01
期刊：
影响因子：
2.1
通讯作者：
Chibana, Hiroji
中科院分区：
文献类型：
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作者：
Aoyama, Toshihiro;Nakayama, Hironobu;Chibana, Hiroji
转录组5-侧翼区的DNA测序有效地识别了转录起始点，也有助于识别未知基因。这项研究描述了转录起始点的全面轮询和来自致病真菌光滑假丝酵母基因组的全长互补DNA的分析。将从不同培养条件下生长的细胞制备的cDNA文库的序列读数与念珠菌基因组数据库(cgd：http://www.candidagenome.org/))的参考基因组序列进行比较，发现了4316个基因的表达及其公认的转录起始点(TSS)。此外，这项分析还预测了59个新基因，其中22个与酿酒酵母的基因组没有同源性，酿酒酵母是光滑酵母的遗传近亲。此外，对光肩星天牛和酿酒酵母的5-非翻译区(5-UTRs)和核心启动子进行了比较，发现不同的同源基因具有不同的相似性和差异性。因此，光肩星毛虫转录组可以补充基因组数据库的注释，并应该为这种重要的人类病原体的基因的组织、调节和功能提供新的见解。
DNA sequencing of the 5-flanking region of the transcriptome effectively identifies transcription initiation sites and also aids in identifying unknown genes. This study describes a comprehensive polling of transcription start sites and an analysis of full-length complementary DNAs derived from the genome of the pathogenic fungus Candida glabrata. A comparison of the sequence reads derived from a cDNA library prepared from cells grown under different culture conditions against the reference genomic sequence of the Candida Genome Database (CGD: http://www.candidagenome.org/) revealed the expression of 4316 genes and their acknowledged transcription start sites (TSSs). In addition this analysis also predicted 59 new genes including 22 that showed no homology to the genome of Saccharomyces cerevisiae, a genetically close relative of C.glabrata. Furthermore, comparison of the 5-untranslated regions (5-UTRs) and core promoters of C.glabrata to those of S.cerevisiae showed various global similarities and differences among orthologous genes. Thus, the C.glabrata transcriptome can complement the annotation of the genome database and should provide new insights into the organization, regulation, and function of genes of this important human pathogen.