NATURAL HEMOLYTIC AND BACTERICIDAL ACTIVITIES OF SEA BREAM SPARUS-AURATA SERUM ARE EFFECTED BY THE ALTERNATIVE COMPLEMENT PATHWAY
NATURAL HEMOLYTIC AND BACTERICIDAL ACTIVITIES OF SEA BREAM SPARUS-AURATA SERUM ARE EFFECTED BY THE ALTERNATIVE COMPLEMENT PATHWAY
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DOI：
10.1016/0165-2427(94)05430-z
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发表时间：
1995-04-01
影响因子：
1.8
通讯作者：
TORT, L
中科院分区：
文献类型：
--
作者：
SUNYER, JO;TORT, L
海鲷血清具有杀菌和溶血活性。当血清与补体旁路途径 (ACP) 的不同激活剂一起孵育时，这些活性就会减弱。乙二胺四乙酸(EDTA)同时抑制溶血和杀菌活性，而乙二醇-双(B-氨基乙基醚)-N,N,N'-四乙酸(EGTA)则没有抑制作用。产生了针对假定的鲷鱼成分第三种成分（C3）的抗体。免疫电泳观察发现，鲷鱼C3与人C3迁移位置一致。交叉免疫电泳表明，与人类对应物相比，鲷鱼 C3 表现出相似的激活模式。抗鲷鱼C3抗体同时抑制杀菌和溶血活性。结论是两种血清作用均由 ACP 显示。研究了鲷鱼 ACP 滴定的最佳条件。在所有测试的哺乳动物红细胞中，兔红细胞 (RaRBC) 被发现是最好的 ACP 激活剂，因此用于滴定。与哺乳动物相比，海鲷显示出非常高的 ACP 滴度。天然存在的抗兔 RaRBC 抗体的吸收不影响 ACP 滴度。从不同哺乳动物红细胞中酶促去除唾液酸增加了这些细胞对由海鲷 ACP 介导的溶血的敏感性。
Sea bream serum displayed bactericidal and hemolytic activities. These activities were depleted when serum was incubated with different activators of the alternative complement pathway (ACP). Ethylenediaminetetraacetic acid (EDTA) inhibited both the hemolytic and bactericidal activities, while ethyleneglycol-bis (B-aminoethyl ether)-N,N,N'-tetraacetic acid (EGTA) was not inhibitory. An antibody against the putative third component of sea bream component (C3) was produced. It was observed by immunoelectrophoresis that the sea bream C3 and human C3 migrated in the same position. Crossed immunoelectrophoresis showed that sea bream C3 exhibited a similar pattern of activation when compared with its human counterpart. The anti-sea bream C3 antibody inhibited both bactericidal and hemolytic activities. It was concluded that both serum actions were displayed by the ACP. The best conditions for the sea bream ACP titration were investigated. Of all mammal erythrocytes tested, rabbit erythrocytes (RaRBC) were found to be the best ACP activators and thus were used for the titration. Sea bream showed very high ACP titers when compared with those of mammals. Absorption of naturally occurring antibodies against rabbit RaRBC did not influence the ACP titers. Enzymatic removal of sialic acid from different mammalian erythrocytes increased the sensitivity of these cells to hemolysis mediated by the sea bream ACP.