Elevated concentrations of human neutrophil peptides in plasma, blood, and body fluids from patients with infections
Elevated concentrations of human neutrophil peptides in plasma, blood, and body fluids from patients with infections
复制标题
DOI：
10.1086/516075
复制
发表时间：
1997-11-01
影响因子：
11.8
通讯作者：
Matsukura, S
中科院分区：
文献类型：
--
作者：
Ihi, T;Nakazato, M;Matsukura, S
神经肽，也称为防御素，是位于嗜中性粒细胞的嗜天青颗粒中的抗微生物分子。我们使用一种灵敏的放射免疫测定法来测量86名健康志愿者作为对照和54名各种感染患者的血浆和血液中人中性粒细胞肽（HNPs）1-3的浓度。在细菌感染、非细菌感染和肺结核发作时，患者中HNPs 1-3的相应平均血浆浓度分别是对照组平均值（+/- SE）（254.8 +/- 7.1 pg/mu L）的4.2、3.2和1.8倍。患者的HNP 1-3的血浆浓度与外周血中性粒细胞计数相关（相关系数= 0.629; P = .0001）。细菌感染患者血液HNPs 1-3的平均浓度（+/- SE）高于对照组的平均值（+/- SE）（10.4 +/- 0.6 ng/μ L），而非细菌感染或结核病患者的平均浓度（+/- SE）与对照组无差异。来自细菌感染患者的血浆样品的反相高效液相色谱显示高浓度的HNP 1-3的两种前体，表明中性粒细胞前体细胞中HNP的生物合成响应于感染而被刺激和/或pro-HNP的释放被增强。感染患者的胸腔液、支气管肺泡灌洗液、尿液和脑脊液中的HNPs 1-3浓度也升高。我们的研究结果表明，HNP在感染中具有生理意义，并且该肽家族可能有助于评估感染期间中性粒细胞的功能。
Neutrophil peptides, also called defensins, are antimicrobial molecules localized in the azurophil granules of neutrophils. We used a sensitive radioimmunoassay to measure the concentrations of human neutrophil peptides (HNPs) 1-3 in the plasma and blood of 86 healthy volunteers who served as controls and 54 patients with various infections. The respective mean plasma concentrations of HNPs 1-3 in the patients at the onset of bacterial infection, nonbacterial infection, and pulmonary tuberculosis were 4.2, 3.2, and 1.8 times the mean value (+/- SE) for the controls (254.8 +/- 7.1 pg/mu L). Plasma concentrations of HNPs 1-3 for the patients were correlated with the peripheral blood neutrophil counts (correlation coefficient = 0.629; P = .0001). The mean concentration (+/- SE) of blood HNPs 1-3 in patients with bacterial infections was higher than the mean value (+/- SE) for controls (10.4 +/- 0.6 ng/mu L), whereas the mean concentration (+/- SE) in patients with nonbacterial infections or tuberculosis did not differ from that for controls. Reverse-phase high performance liquid chromatography of plasma samples from patients with bacterial infections showed high concentrations of two precursors of HNPs 1-3, indicating that the biosynthesis of HNPs in neutrophil precursor cells is stimulated in response to infection and/or that the release of pro-HNPs is enhanced. HNPs 1-3 concentrations in the pleural fluid, bronchoalveolar lavage fluid, urine, and cerebrospinal fluid were also elevated in patients with infections. Our results suggest that HNPs have physiological significance in infection and that this family of peptides may be useful in assessing neutrophil function during infection.