Identification of hepatitis A virus mimotopes by phage display, antigenicity and immunogenicity
Identification of hepatitis A virus mimotopes by phage display, antigenicity and immunogenicity
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DOI：
10.1016/j.jviromet.2006.10.015
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发表时间：
2007-03-01
影响因子：
3.1
通讯作者：
Perez, Ela M.
中科院分区：
文献类型：
--
作者：
Larralde, Osmany G.;Martinez, Raiza;Perez, Ela M.
利用噬菌体展示肽技术首次筛选出模拟甲型肝炎病毒抗原决定簇的配体。利用噬菌体展示肽库，通过对甲型肝炎患者血清抗体的亲和筛选，获得了展示甲型肝炎病毒模拟表位的噬菌体。筛选选择的多肽与HAV感染患者和健康对照血清的反应性。4个不同序列的克隆肽被鉴定为HAV的模拟表位，其中3个肽的氨基酸序列与HAV的VP3和VP1抗原蛋白中的至少一个相似。一个克隆被92%的阳性血清识别。通过ELISA测定，噬菌体有效地与HAV竞争吸收人血清中的抗HAV特异性抗体。四个噬菌体克隆在免疫小鼠中诱导中和抗HAV抗体。的：结果表明，这种方法的潜力，阐明疾病相关的抗原表位的HAV和使用这些模拟表位在诊断应用或在开发基于模拟表位的甲型肝炎疫苗，而不需要操纵的病毒。(c)2006 Elsevier B.V.保留所有权利。
A phage-displayed peptide approach was used to identify ligands mimicking antigenic determinants of hepatitis A virus (HAV) for the first time. Bacteriophages displaying HAV mimotopes were isolated front a phage-display peptide library by affinity selection on Serum antibodies from hepatitis A patients. Selected pliage-peptides were screened for reactivity with sera from HAV infected patients and healthy controls. Four cloned peptides with different sequences were identified as mimotopes of HAV: three of them showed similarity in their amino acid sequences with at least one of the VP3 and VP1 antigenic proteins of HAV. One clone was recognised by 92% of the positive sera. The phagotopes competed effectively with HAV for absorption of anti-HAV-specific antibodies in human sera, as determined by ELISA. The four phage clones induced neutralising anti-HAV antibodies in immunised mice. The: e results demonstrate the potential of this method to elucidate the disease related epitopes of HAV and to use these mimotopes in diagnostic applications or in the development of a mimotope-based hepatitis A vaccine without the necessity of manipulation of the virus. (c) 2006 Elsevier B.V. All rights reserved.