miR-663a inhibits hepatocellular carcinoma cell proliferation and invasion by targeting HMGA2
miR-663a inhibits hepatocellular carcinoma cell proliferation and invasion by targeting HMGA2
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DOI：
10.1016/j.biopha.2016.04.034
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发表时间：
2016-07-01
影响因子：
7.5
通讯作者：
Yuan, Xia
中科院分区：
文献类型：
--
作者：
Huang, Weizhen;Li, Jun;Yuan, Xia
肝细胞癌（HCC）是全世界范围内一种高度侵袭性的实体恶性肿瘤。 miRNA 失调通过细胞增殖、凋亡和转移的异常调节在 HCC 进展中发挥重要作用。 miR-663a 是一种研究很少的 miRNA。 miR-663a 是否调节 HCC 发展仍不清楚。该研究的目的是探讨 miR-663a 在 HCC 发展中的作用。为了确定 HCC 中 miR-663a 的表达水平，我们分析了 GSE21362 和 TCGA 的数据。结果显示，与邻近非肿瘤组织相比，miR-663a在HCC组织中显着下调。功能获得和功能丧失测定表明，miR-663a 明显抑制细胞增殖、迁移和侵袭。机制研究表明，miR-663a 通过靶向和抑制高迁移率基团 A2 (HMGA2) 来调节细胞功能。此外，HMGA2 的过表达显着减弱了 miR-663a 的肿瘤抑制作用。综上所述，miR-663a 通过靶向 HMGA2 抑制 HCC 细胞增殖和运动。 (C) 2016 年由 Elsevier Masson SAS 出版。
Hepatocellular carcinoma (HCC) is a highly aggressive solid malignancy throughout the world. Dysregulation of miRNAs play essential roles in HCC progression via aberrant regulation of cell proliferation, apoptosis, as well as metastasis. miR-663a is a poorly investigated miRNA. Whether miR-663a regulates HCC development remains unknown. The aim of the study was to explore the role of miR-663a in HCC development. To determine the expression level of miR-663a in HCC, we analyzed the data from GSE21362 and TCGA. The results showed that miR-663a was significantly down-regulated in HCC tissue compared with adjacent non-tumor tissue. Gain of function and loss of function assays revealed that miR-663a distinctly inhibited cell proliferation, migration and invasion. Mechanistic investigations demonstrated that miR-663a modulated cell functions through targeting and suppressing high mobility group A2 (HMGA2). In addition, overexpression of HMGA2 remarkably attenuated the tumor repressive effect of miR-663a. Taken together, miR-663a inhibits HCC cell proliferation and motility by targeting HMGA2. (C) 2016 Published by Elsevier Masson SAS.