High-affinity folate binding in human prostate
High-affinity folate binding in human prostate
复制标题
人前列腺中的高亲和力叶酸结合
DOI：
10.1007/bf01145962
复制
发表时间：
1993
影响因子：
4
通讯作者：
M. Høier‐Madsen
中科院分区：
文献类型：
--
作者：
J. Holm;S. Hansen;M. Høier‐Madsen
Binding of3H-folate in Triton X-100 solubilized human prostate homogenate was of a high-affinity type and displayed apparent positive cooperativity typical of specific folate binding. Radioligand dissociation was slow at pH 7.4, but rapid at pH 3.5. Gel chromatography reveled two major folate binding proteins (Mr≈100 and 25kDa), but only one single band (Mr≈65–70 kDa) was detectable on SDS-PAGE and immunoblotting with rabbit-anti human milk folate binding protein. Concentration of folate binding protein in prostate homogenate expressed as maximum3H-folate binding was 1.10 nmol/g protein, and the cross-reactivity with rabbit-anti human milk folate binding protein serum was 15% as determined by an enzyme-linked immunosorbent assay (median values; n=6).
