Regulation of transcripts encoding ADAMTS-1 (a disintegrin and metal loproteinase with thrombospondin-like motifs-1) and progesterone receptor by human chorionic gonadotropin in equine preovulatory follicles
Regulation of transcripts encoding ADAMTS-1 (a disintegrin and metal loproteinase with thrombospondin-like motifs-1) and progesterone receptor by human chorionic gonadotropin in equine preovulatory follicles
复制标题
DOI：
10.1677/jme.0.0310473
复制
发表时间：
2003-12-01
影响因子：
3.5
通讯作者：
Sirois, J
中科院分区：
文献类型：
--
作者：
Boerboom, D;Russell, DL;Sirois, J
具有血小板反应蛋白样基序的去整合素和金属蛋白酶-1（ADAMTS-1）是一个新的金属蛋白酶家族的成员，已发现其在排卵的啮齿动物卵泡的颗粒细胞中表达并被生殖诱导。此外，ADAMTS-1基因的靶向破坏导致与生育力严重受损相关的卵巢缺陷。虽然这些数据证明了ADAMTS-1在啮齿动物卵巢生理学中的重要性，但ADAMTS-1在单排卵物种的排卵过程中的潜在作用仍然未知。本研究的目的是克隆马ADAMTS-1初级转录本，并研究其在人绒毛膜促性腺激素（hCG）诱导排卵过程中的调控。一个3573 bp的卵泡cDNA文库克隆被分离出来，并发现编码一个几乎完整的，高度保守的ADAMTS-1同源。实时RT-PCR分析在不同组织中检测到这种转录本，包括以前未报道的ADAMTS-1表达位点，如男性生殖道，卵泡内膜和成熟黄体。孕激素受体（PR），一个已知的调节ADAMTS-1的表达在啮齿动物排卵前卵泡的组织分布，被发现重叠的ADAMTS-1在某些组织中。在hCG诱导的排卵过程中，对卵泡ADAMTS-1和PR mRNA的调节的研究揭示了颗粒细胞和内膜中不同的调节模式。在颗粒细胞中，hCG后12 h ADAMTS-1 mRNA被诱导表达（P
One member of a new family of metalloproteinases, a disintegrin and metalloproteinase with thrombospondin-like motifs-1 (ADAMTS-1), has been found to be expressed and hormonally induced in granulosa cells of ovulating rodent follicles. Furthermore, the targeted disruption of the ADAMTS-1 gene resulted in ovarian defects associated with severely impaired fertility. While these data demonstrate the importance of ADAMTS-1 in rodent ovarian physiology, the potential role of ADAMTS-1 in the ovulatory process of monoovulatory species remains unknown. The objectives of this study were to clone the equine ADAMTS-1 primary transcript and to study its regulation during human chorionic gonadotropin (hCG)-induced ovulation. A 3573 bp follicular cDNA library clone was isolated and found to encode a nearly complete, highly conserved ADAMTS-1 homologue. Real-time RT-PCR analysis detected this transcript in diverse tissues, including previously unreported sites of ADAMTS-1 expression such as the male reproductive tract, the follicular theca interna and the mature corpus luteum. The tissue distribution of the progesterone receptor (PR), a known regulator of ADAMTS-1 expression in rodent preovulatory follicles, was found to overlap that of ADAMTS-1 in some tissues. A study of the regulation of follicular ADAMTS-1 and PR mRNAs during the hCG-induced ovulatory process revealed distinct patterns of regulation in granulosa cells and in theca interna. In granulosa cells, ADAMTS-1 mRNA was found to be induced at 12 h post-hCG (P