Association between naturally occurring spine osteoarthritis in geriatric rats and neurogenic inflammation within neurosegmentally linked skeletal muscle
Association between naturally occurring spine osteoarthritis in geriatric rats and neurogenic inflammation within neurosegmentally linked skeletal muscle
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DOI：
10.1016/j.exger.2019.01.002
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发表时间：
2019-04-01
影响因子：
3.9
通讯作者：
Srbely, John Z.
中科院分区：
文献类型：
--
作者：
Duarte, Felipe C. K.;Hurtig, Mark;Srbely, John Z.
目的：本研究旨在研究老年大鼠与年轻对照组中自然发生的脊柱骨关节炎（OA）（L3-L5）、中枢（L4-L5）P物质（SP）表达和神经节段连接的四头肌（L2-L5）内神经源性炎症的存在之间的关联。设计：对8只老年（27 +/- 3.2个月）和6只年轻（4 +/- 0.0个月）雄性Wistar京都大鼠实施安乐死，并进行微型计算机断层扫描以确定脊柱OA。SP表达（%面积）在脊髓背角，以及SP和蛋白酶激活受体2（PAR 2）的相对表达，以α-微管蛋白在股四头肌muscle测定免疫组化和Western Blot.Results：脊柱骨关节炎被证实在所有老年大鼠，但没有年轻的控制。老年大鼠脊髓背角SP蛋白表达明显增加（MD = 0.086; 95% CI [0.026至0.145]; p = 0.0094）和四头肌（MD = 1.209; 95% CI [0.239 - 2.179]; p = 0.0191）和PAR 2（MD = 0.797; 95% CI [0.160至1.435]; p = 0.0187）。这些观察结果为神经源性炎症在老年人群中自然发生的脊柱OA的肌筋膜疼痛综合征的病理生理学中的潜在作用提供了新的见解。
Objective: This study aimed to investigate the association between naturally occurring spinal osteoarthritis (OA) (L3-L5), the expression of substance P (SP) centrally (L4-L5) and the presence of neurogenic inflammation within the neurosegmentally linked quadriceps (L2-L5) in elderly rats versus young controls.Design: Eight aged (27 +/- 3.2 months) and six young (4 +/- 0.0 months) male Wistar Kyoto rats were euthanized and submitted to micro-computerized tomography for determination of spine OA. SP expression (% area) at the dorsal horn of the spinal cord as well as the relative expression of SP and protease-activated receptor 2 (PAR2) to alpha-tubulin within quadriceps muscle were determined by immunohistochemistry and Western Blot.Results: Spine osteoarthritis was confirmed in all aged rats but no young controls. Aged rats expressed significant increase of SP protein expression within the dorsal horn (MD = 0.086; 95% CI [0.026 to 0.145]; p = 0.0094) and quadriceps (MD = 1.209; 95% CI [0.239 to 2.179]; p = 0.0191) and PAR2 (MD = 0.797; 95% CI [0.160 to 1.435]; p = 0.0187) compared to young controls.Conclusion: These observations provide novel insight into the potential role of neurogenic inflammation in the pathophysiology of myofascial pain syndrome in the naturally occurring spinal OA in elderly population.