ARTICULAR-CARTILAGE VESICLES GENERATE CALCIUM PYROPHOSPHATE DIHYDRATE-LIKE CRYSTALS INVITRO
ARTICULAR-CARTILAGE VESICLES GENERATE CALCIUM PYROPHOSPHATE DIHYDRATE-LIKE CRYSTALS INVITRO
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DOI：
10.1002/art.1780350218
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发表时间：
1992-02-01
影响因子：
--
通讯作者：
RYAN, LM
中科院分区：
文献类型：
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作者：
DERFUS, BA;RACHOW, JW;RYAN, LM
Objective. 鉴定成年猪透明关节软骨消化物的矿物质形成部分的形态并表征其形成的矿物质。 电子显微镜、傅里叶变换红外光谱（FTIR）、X射线显微分析、补偿偏振光显微镜和生化研究，包括C-14标记的UDPG焦磷酸水解酶放射性测定。 关节软骨消化物的该部分含有类似于生长板软骨基质囊泡的膜限制性囊泡，并且在含有ImM ATP的生理盐溶液中仅24小时后就形成矿物质。 矿物质含有无机焦磷酸盐，其中95%来自ATP，和磷酸盐，其中93%来自培养基中的无机磷酸盐。 这种矿物的FTIR光谱与标准焦磷酸钙二水合物（CPPD）晶体的光谱非常相似。 补偿偏振光显微镜显示正双折射，棒状晶体形态相同的CPPD。 钙磷比，定义的能量色散微量分析，也与CPPD一致。 猪透明软骨的关节软骨囊泡部分能够产生与CPPD非常相似的矿物质。
Objective. To identify the morphology of a mineral-forming fraction of adult porcine hyaline articular cartilage digest and characterize the mineral it forms.Methods. Electron microscopy, Fourier transform infrared (FTIR) spectroscopy, x-ray microanalysis, compensated polarized light microscopy, and biochemical studies including C-14-labeled UDPG pyrophosphohydrolase radiometric assay.Results. This fraction of articular cartilage digest contained membrane-limited vesicles resembling growth plate cartilage matrix vesicles and formed mineral after only 24 hours in physiologic salt solution containing 1 mM ATP. The mineral contained inorganic pyrophosphate, 95% of which derived from ATP, and phosphate, 93% of which derived from inorganic phosphate in the medium. The FTIR spectrum of this mineral closely resembled the spectrum of standard calcium pyrophosphate dihydrate (CPPD) crystals. Compensated polarized light microscopy showed positively birefringent, rod-shaped crystals morphologically identical to CPPD. Ca:P ratios, defined by energy-dispersive microanalysis, were also consistent with CPPD.Conclusion. The articular cartilage vesicle fraction of porcine hyaline cartilage is capable of generating mineral that strongly resembles CPPD.