Phylogenetic Diversity of Chytridiomycetes in a Temporary Forest Pond Surveyed using Culture-Based Methods
Phylogenetic Diversity of Chytridiomycetes in a Temporary Forest Pond Surveyed using Culture-Based Methods
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DOI：
10.1656/058.015.0319
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发表时间：
2016-09-01
影响因子：
0.6
通讯作者：
Powell, Martha J.
中科院分区：
文献类型：
--
作者：
Davis, William J.;Antonetti, Jonathan;Powell, Martha J.
临时森林池塘是每年在非淹没和淹没阶段之间循环的洼地，被认为是两栖动物和其他类群的生物多样性热点。然而，人们对临时森林池塘的微生物群落知之甚少，对壶菌（一种不显眼的、早期分化的真菌谱系，存在于此类池塘中）知之甚少。我们从塔拉迪加国家森林橡树马尔吉区的一个临时森林池塘中采集了土壤和水样。使用诱饵和标准分离技术从样品中分离壶菌。使用基于核大亚基 rDNA 比对推断的分子系统发育的单系物种概念来鉴定分离菌株。共分离出30株菌株，代表7个种。由于基于培养的方法在能够培养所有存在的壶菌方面存在局限性，我们仅描述了壶菌群落的一部分。然而，这项研究为未来的清单奠定了基础，未来应用多相方法进一步揭示这个尚未充分研究和变化的栖息地中的壶菌多样性。
Temporary forest ponds are depressions that annually cycle between non-inundated and inundated stages and are recognized as biodiversity hotspots for amphibians and other taxa. However, little is known about the microbial communities of temporary forest ponds and less is known about chytrids, an inconspicuous, early diverging lineage of fungi, present in such ponds. We sampled soil and water from a temporary forest pond in the Oak-mulgee District of the Talladega National Forest. Chytrids were isolated from the samples using baits and standard isolating techniques. Isolated strains were identified using the monophyletic-species concept based on a molecular phylogeny inferred from an alignment of the nuclear large subunit rDNA. A total of 30 strains were isolated, which represented 7 species. Due to the limitations of a culture-based method in being able to culture all chytrids that are present, we have described only a portion of the chytrid community. This study, however, is foundational to future inventories that apply a multiphasic approach to further reveal chytrid diversity in this understudied and variable habitat.