Highly efficient electrochemiluminescence labels comprising iridium(iii) complexes.
Highly efficient electrochemiluminescence labels comprising iridium(iii) complexes.
复制标题
DOI：
10.1039/c6dt04038a
复制
发表时间：
2017-01
影响因子：
4
通讯作者：
Yuyang Zhou;Kai Xie;Ruimei Leng;Lingyan Kong;Chengbao Liu;Qingqing Zhang;Xiaomei Wang
中科院分区：
文献类型：
--
作者：
Yuyang Zhou;Kai Xie;Ruimei Leng;Lingyan Kong;Chengbao Liu;Qingqing Zhang;Xiaomei Wang
提高标记剂的性能是电化学发光（ECL）相关技术进一步发展的关键。尽管目前已经报道了大量的钌配合物以外的ECL发光体，但涉及新型ECL标记剂的研究仍然很少。在本文中，五个新的铱ECL标签和一个控制标签，包括钌络合物已被合理地设计和彻底的特征在于使用电子物理技术，理论计算和电化学表征，以评估在这项工作中的ECL性能。无论是在核反应器辅助电位扫描实验，并通过湮灭的方法，大多数这些新的铱标签表现出等效或甚至更强的ECL信号比三-2，2 '-联吡啶钌（ii）[简称为Ru（bpy）32+]在相同的实验条件下。最重要的是，使用相同的标记方案，在ProCell缓冲溶液中，用铱标记物（本工作中的标记物3）标记的牛血清白蛋白（BSA）表现出比用传统钌络合物（本工作中的Ru基标记物）标记的相同量强得多的ECL信号（1.9倍）。除了表现出各种发射颜色的优势外，这项工作中报道的新型铱标记物可能为基于ECL的分析技术的进一步发展提供见解。
Improving the performance of labeling agents is crucial to the further development of electrochemiluminescence (ECL) related technologies. Although a large number of ECL luminophores beyond ruthenium complexes have been reported so far, there is still a scarcity of studies involving novel ECL labeling agents. Herein, five novel iridium ECL labels and one control label comprising a ruthenium complex have been rationally designed and thoroughly characterized using photophysical techniques, theoretical calculations and electrochemical characterization to assess the ECL properties in this work. Both in coreactant-assisted potential scan experiments and through an annihilation approach, most of these novel iridium labels exhibit equivalent or even-stronger ECL signals than tri-2,2'-bipyridylruthenium(ii) [abbreviated as Ru(bpy)32+] under the same experimental conditions. Most importantly, using the same labeling protocol, bovine serum albumin (BSA) labeled with an iridium label (label 3 in this work) exhibits a much more intense ECL signal (1.9 times) than the same amount labeled with a traditional ruthenium complex (Ru-based Label in this work) in ProCell buffer solution. In addition to the advantage of exhibiting various emission colors, the novel iridium labels reported in this work may offer insight for the further development of ECL-based analytical technology.