MODULATION OF MURINE IMMUNOLOGICAL RESPONSES BY SALIVARY-GLAND EXTRACT OF SIMULIUM-VITTATUM (DIPTERA, SIMULIIDAE)
MODULATION OF MURINE IMMUNOLOGICAL RESPONSES BY SALIVARY-GLAND EXTRACT OF SIMULIUM-VITTATUM (DIPTERA, SIMULIIDAE)
复制标题
DOI：
10.1093/jmedent/30.5.928
复制
发表时间：
1993-09-01
影响因子：
2.1
通讯作者：
ENRIQUEZ, FJ
中科院分区：
文献类型：
--
作者：
CROSS, ML;CUPP, MS;ENRIQUEZ, FJ
用小鼠模型宿主(实验小鼠)研究了刺参唾液腺提取物对几种免疫机制的影响。这些机制包括主要组织相容性复合体II类细胞表面分子的表达，淋巴细胞的体外有丝分裂原反应性，以及对异源异源抗原(绵羊红细胞)的抗体反应。实验旨在确定唾液腺提取物在体内接种或体外包涵体后对细胞培养的影响。体内接种唾液腺提取物可降低脾细胞群中Ia+细胞的百分比，但无论唾液腺提取物是否包括在培养物中，这种差异都可以通过每IoD体外培养2d而得到改善。唾液腺提取物对来源于区域淋巴结或皮肤(表皮)的细胞的Ia表达无影响。在体内接种唾液腺提取物不影响脾淋巴细胞对有丝分裂原的反应性，而在体外接触唾液腺提取物则减少T和B细胞的有丝分裂。最后，如果将唾液腺提取物作为共接种剂，绵羊红细胞的抗体反应会增强，尽管这只在系统水平上表达，而不考虑抗原传递的途径。根据这些结果，推测了黑蝇唾液的免疫调节功能；它们可能通过细胞因子调节，在不同水平上对免疫系统的不同亚室起作用。
The influence of Simulium vittatum Zetterstedt salivary gland extract on several immunological mechanisms was investigated in murine model hosts (laboratory mice). These mechanisms included the expression of major histocompatibility complex class II cell surface molecules, the in vitro mitogen responsiveness of lymphoid cells, and the antibody responses to heterologous foreign antigens (sheep erythrocytes). Experiments were designed to determine the influence of salivary gland extract following in vivo inoculation or in vitro inclusion in cell culture. In vivo inoculation of salivary gland extract reduced the percentage of Ia+ cells in spleen cell populations, although this difference was ameliorated by a 2 d in vitro culture per-iod, regardless of whether salivary gland extract was included in culture. Salivary gland extract had no effect on Ia expression by cells derived from regional lymph nodes or the skin (epidermis). In vivo inoculation with salivary gland extract did not affect the responsiveness of splenic lymphocytes to mitogens, whereas in vitro exposure to salivary gland extract reduced both T and B cell mitogenesis. Finally, antibody responses to sheep erythrocytes were enhanced if salivary gland extract was included as a coinoculant, although this was expressed only at the systemic level regardless of the route of antigen delivery. In light of these results, immunomodulatory functions of black fly saliva are postulated; they are operative at different levels on different subcompartments of the immune system, possibly via cytokine modulation.