Comparison of three tracers for detecting lung epithelial injury in anesthetized sheep.
Comparison of three tracers for detecting lung epithelial injury in anesthetized sheep.
复制标题
三种示踪剂检测麻醉羊肺上皮损伤的比较。
DOI：
10.1152/jappl.1989.66.5.2374
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Holiday,DB
中科院分区：
文献类型：
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作者：
Peterson,BT;Dickerson,KD;James,HL;Miller,EJ;McLarty,JW;Holiday,DB
我们比较了三种雾化示踪剂区分对照组、呼气末正压为10 cmH 2 O的肺充气组、无肺损伤的肺血管性高血压和水肿组以及因静脉注射油酸导致的肺损伤的肺水肿组的能力。示踪剂为99 mTc-二亚乙基三胺五乙酸盐（99 mTc-DTPA，mol wt 492）、99 mTc-人血清白蛋白（99 mTc-ALB，mol wt 69，000）和99 mTc-聚集白蛋白（99 mTc-AGG ALB，mol wt 383，000）。99 mTc-DTPA清除率测量不能区分肺损伤和肺膨胀。99 mTc-AGG ALB清除率不受肺膨胀的影响，并随肺损伤而略有增加。99 mTc-ALB清除率（0.06 +/- 0.02%/min）与肺充气（0.09 +/- 0.02%/min，P> 0.05）或无损伤高血压4 h（0.09 +/- 0.04%/min，P> 0.05）无变化。油酸15 min内99 mTc-ALB的沉积使清除率增加至0.19 ± 0.06%/min，与死后肺水体积呈良好的相关性（r = 0.92，P <0.01）。这并没有发生时，有一个60分钟的延迟沉积的99 mTc-ALB。我们得出结论，99 mTc-ALB是最好的指标，用于研究肺上皮损伤的影响，蛋白质和液体运输进出的空气空间的肺在微创的方式。
We compared the ability of three aerosolized tracers to discriminate among control, lung inflation with a positive end expired pressure of 10 cmH2O, lung vascular hypertension and edema without lung injury, and lung edema with lung injury due to intravenous oleic acid. The tracers were 99mTc-diethylenetriaminepentaacetate (99mTc-DTPA, mol wt 492), 99mTc-human serum albumin (99mTc-ALB, mol wt 69,000), and 99mTc-aggregated albumin (99mTc-AGG ALB, mol wt 383,000). 99mTc-DTPA clearance measurements were not able to discriminate lung injury from lung inflation. The 99mTc-AGG ALB clearance rate was unchanged by lung inflation and increased slightly with lung injury. The 99mTc-ALB clearance rate (0.06 +/- 0.02%/min) was unchanged by lung inflation (0.09 +/- 0.02%/min, P greater than 0.05) or 4 h of hypertension without injury (0.09 +/- 0.04%/min, P greater than 0.05). Deposition of 99mTc-ALB within 15 min of the administration of the oleic acid increased the clearance rate to 0.19 +/- 0.06%/min, which correlated well with the postmortem lung water volume (r = 0.92, P less than 0.01). This did not occur when there was a 60-min delay in the deposition of 99mTc-ALB. We conclude that 99mTc-ALB is the best indicator for studying the effects of lung epithelial injury on protein and fluid transport into and out of the air spaces of the lungs in a minimally invasive manner.