Structural gymnastics of multifunctional metamorphic proteins.
Structural gymnastics of multifunctional metamorphic proteins.
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DOI：
10.1007/s12551-011-0053-8
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发表时间：
2011-09-01
影响因子：
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通讯作者：
Brown, Louise J
中科院分区：
文献类型：
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作者：
Goodchild, Sophia C;Curmi, Paul M G;Brown, Louise J
经典的结构-功能范式认为，蛋白质表现出一种单一的定义良好的天然状态，从而产生其生物功能。尽管如此，在过去的几十年里，已经发现了许多由多种不同无序结构状态引起的蛋白质表现出生物功能的例子。最近，几个“变形蛋白”的例子，能够在生理条件下在截然不同的原生样拓扑结构之间相互转换，具有多种功能。在这篇综述中，我们着眼于蛋白质变态的概念与目前对蛋白质结构-功能景观的理解。虽然观察到的结构动力学为变形蛋白的功能多样性提供了一个新的来源，但变形蛋白的动态性通常使它们难以用传统的蛋白质结构测定方法进行识别和探测。然而，随着变态蛋白的存在已经确立，以及分析多种蛋白质构象的技术正在改进，这类蛋白质的数量很可能会继续增长。
The classic structure-function paradigm holds that a protein exhibits a single well-defined native state that gives rise to its biological function. Nonetheless, over the past few decades, numerous examples of proteins exhibiting biological function arising from multiple structural states of varying disorder have been identified. Most recently, several examples of 'metamorphic proteins', able to interconvert between vastly different native-like topologies under physiological conditions, have been characterised with multiple functions. In this review, we look at the concept of protein metamorphosis in relation to the current understanding of the protein structure-function landscape. Although structural dynamism observed for metamorphic proteins provides a novel source of functional versatility, the dynamic nature of the metamorphic proteins generally makes them difficult to identify and probe using conventional protein structure determination methods. However, as the existence of metamorphic proteins has now been established and techniques enabling the analysis of multiple protein conformers are improving, it is likely that this class will continue to grow in number.