STRUCTURAL CHANGES IN ESCHERICHIA COLI INFECTED WITH PHIX174 TYPE BACTERIOPHAGE
STRUCTURAL CHANGES IN ESCHERICHIA COLI INFECTED WITH PHIX174 TYPE BACTERIOPHAGE
复制标题
DOI：
10.1099/0022-1317-5-1-113
复制
发表时间：
1969-01-01
影响因子：
3.8
通讯作者：
ROBERTSON, D
中科院分区：
文献类型：
--
作者：
BRADLEY, DE;DEWAR, CA;ROBERTSON, D
吖啶橙子染色显示α 3噬菌体含有单链DNA，其形态与φ X 174相似。用高倍数感染的大肠杆菌作薄切片，电镜观察其感染过程。潜伏期约为13分钟，但直到感染后10分钟，质膜开始从两极缩回时，在宿主细胞中未见明显的结构变化。在14分钟时，检测到细胞内噬菌体，并且在细胞的中点处出现凸起。在21分钟时，许多细胞以原生质球的形式存在，其他细胞开始溶解。由噬菌体感染形成的原生质球的裂解与青霉素产生的原生质球的裂解不同。
The coliphage α 3, which is morphologically similar to φX174, was shown by acridine orange staining to contain single-stranded DNA. Its infective process was studied by the electron microscopy of thin sections ofEscherichia coliinfected at high multiplicity. The latent period was found to be about 13 min. but no obvious structural changes were visible in host cells until 10 min. after infection, when the plasma membrane began to retract from the poles. At 14 min. intracellular phage was detected and bulges appeared on the cells at their midpoint. At 21 min. many cells were in the form of spheroplasts and others were beginning to lyse. Spheroplasts formed by phage infection lysed differently from those produced by penicillin.