DIFFERENTIAL EXPRESSION OF NUCLEAR HMG1, HMG2 PROTEINS AND H1(0) HISTONE IN VARIOUS BLOOD-CELLS
DIFFERENTIAL EXPRESSION OF NUCLEAR HMG1, HMG2 PROTEINS AND H1(0) HISTONE IN VARIOUS BLOOD-CELLS
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DOI：
10.1002/cbf.290130209
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发表时间：
1995-06-01
影响因子：
3.6
通讯作者：
HRKAL, Z
中科院分区：
文献类型：
--
作者：
CABART, P;KALOUSEK, I;HRKAL, Z
研究了不同类型血细胞、增殖活性和分化阶段中染色体高迁移率族蛋白HMG 1、HMG 2和组蛋白H1（0）水平的变化。通过血细胞5% w/v高氯酸提取物的SDS-PAGE，采用密度测定法评价蛋白质HMG 1、HMG 2和组蛋白H1（0）的相对量。关于HMG 1和HMG 2，主要结论是：这些HMG蛋白在恶性细胞（即白血病细胞系）中的表达高于淋巴细胞或粒细胞，并且HMG 1和HMG 2的分布具有高度的细胞特异性。与淋巴样细胞相比，髓样细胞中HMG 1/2的水平较高。结果表明，在骨髓细胞中，HMG 2优于HMG 1。HMG 1/2表达与细胞增殖活性无直接相关性。HMG 1/2的表达水平也不依赖于染色质的转录。然而，不可逆分化的非增殖细胞和HMG 1/2蛋白的丢失之间存在一些联系。可逆分化的白血病细胞保持其HMG 1/2水平。与HMG 1/2相似，H1（0）显示出强的细胞特异性。H1（0）在不同血细胞类型中的水平不同。与淋巴样细胞相比，髓样细胞中的H1（0）水平高出数倍，无论它们是正常的还是恶性的。此外，在分化的HL-60细胞中存在H1（0）的积累，伴随着细胞增殖的仅轻微下降;这与H1度表达与细胞生长抑制不直接相关的观点一致。而H1（0）的高表达与细胞分化过程中的变化有关。
Changes in the levels of chromosomal high-mobility group proteins HMG1, HMG2 and histone H1(0) were investigated in blood cells of various types, proliferation activity and stage of differentiation. The relative amounts of proteins HMG1, HMG2 and histone H1(0) were evaluated densitometrically by SDS-PAGE of 5 per cent w/v perchloric acid extracts of blood cells. Concerning the HMG1 and HMG2, the main conclusions were: the expression of these HMG proteins was higher in malignant cells, namely leukemia cell lines, then in lymphocytes or granulocytes and the distribution of HMG1 and HMG2 was highly cell-specific. In comparison with lymphoid cells, the levels of HMG1/2 were higher in myeloid cells. The results revealed that in myeloid cells HMG2 prevails over HMG1. There was no direct correlation between HMG1/2 expression and proliferation activity. The levels of HMG1/2 did not depend on the transcription of chromatin either. However, there was some connection between irreversibly differentiated nonproliferating cells and a loss of HMG1/2 proteins. Reversibly differentiated leukemic cells retain their HMG1/2 levels. Similarly to HMG1/2, H1(0) showed a strong cell specificity. The level of H1(0) was different in the various blood cell types. As compared with lymphoid cells, the level of H1(0) was several-fold higher in myeloid cells, regardless of whether they were normal or malignant. Moreover, there was an accumulation of H1(0) in differentiating HL-60 cells accompanied by only a slight decline in cell proliferation; this agrees with the idea that H1 degrees expression is not directly associated with the inhibition of cell growth. Rather higher expression of H1(0) is related to changes during cell differentiation.