The Neuroprotective Effects of Brazilian Green Propolis on Neurodegenerative Damage in Human Neuronal SH-SY5Y Cells.
The Neuroprotective Effects of Brazilian Green Propolis on Neurodegenerative Damage in Human Neuronal SH-SY5Y Cells.
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巴西绿蜂胶对人神经元 SH-SY5Y 细胞神经退行性损伤的神经保护作用
DOI：
10.1155/2017/7984327
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发表时间：
2017
影响因子：
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通讯作者：
Nakanishi H
中科院分区：
文献类型：
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作者：
Ni J;Wu Z;Meng J;Zhu A;Zhong X;Wu S;Nakanishi H
氧化应激和突触功能障碍是与阿尔茨海默病（AD）认知功能障碍相关的主要神经退行性损害。我们已经发现巴西绿色草（brazilian green salt，简称绿草）可以改善生活在高海拔地区的轻度认知障碍患者的认知功能;然而，绿草的作用机制尚不清楚。在本研究中，我们调查的氧化应激，脑源性神经营养因子（BDNF）的表达，和活性调节的细胞因子相关蛋白（Arc），突触效能的关键因素，使用人神经母细胞瘤SH-SY 5 Y细胞的影响。用过氧化氢（H_2O_2 ~-）预处理SH-SY 5 Y细胞，可明显减轻H_2O_2 ~-对SH-SY 5 Y细胞的毒性。此外，在SH-SY 5 Y细胞中，RPF显著减少H2 O2产生的来自线粒体和8-氧代-2 ′-脱氧鸟苷（8-oxo-dG，DNA氧化损伤标记物）的活性氧（ROS），但显著逆转纤维状β-淀粉样蛋白和IL-1β损伤的BDNF诱导的Arc表达。此外，BDNF mRNA的表达显着上调，在时间和剂量依赖性的方式。此外，顺铂通过磷酸肌醇-3激酶（PI 3 K）诱导Arc mRNA和蛋白表达。这些观察结果强烈表明，芦荟通过各种抗氧化剂的特性保护神经元免受神经退行性损伤。本研究提供了巴西绿色枸杞预防AD认知功能障碍和衰老的潜在分子机制。
Oxidative stress and synapse dysfunction are the major neurodegenerative damage correlated to cognitive impairment in Alzheimer's disease (AD). We have found that Brazilian green propolis (propolis) improves the cognitive functions of mild cognitive impairment patients living at high altitude; however, mechanism underlying the effects of propolis is unknown. In the present study, we investigated the effects of propolis on oxidative stress, expression of brain-derived neurotrophic factor (BDNF), and activity-regulated cytoskeleton-associated protein (Arc), the critical factors of synapse efficacy, using human neuroblastoma SH-SY5Y cells. Pretreatment with propolis significantly ameliorated the hydrogen peroxide- (H2O2-) induced cytotoxicity in SH-SY5Y cells. Furthermore, propolis significantly reduced the H2O2-generated reactive oxygen species (ROS) derived from mitochondria and 8-oxo-2′-deoxyguanosine (8-oxo-dG, the DNA oxidative damage marker) but significantly reversed the fibrillar β-amyloid and IL-1β-impaired BDNF-induced Arc expression in SH-SY5Y cells. Furthermore, propolis significantly upregulated BDNF mRNA expression in time- and dose-dependent manners. In addition, propolis induced Arc mRNA and protein expression via phosphoinositide-3 kinase (PI3K). These observations strongly suggest that propolis protects from the neurodegenerative damage in neurons through the properties of various antioxidants. The present study provides a potential molecular mechanism of Brazilian green propolis in prevention of cognitive impairment in AD as well as aging.