"Missing" protists:: a molecular prospective
"Missing" protists:: a molecular prospective
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DOI：
10.1007/s10531-007-9250-y
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发表时间：
2008-02-01
影响因子：
3.4
通讯作者：
Lopez-Garcia, Purificacion
中科院分区：
文献类型：
--
作者：
Epstein, Slava;Lopez-Garcia, Purificacion
基于18S rRNA扩增和测序的分子生态学方法揭示了迄今为止在每个采样环境中微生物真核生物的惊人多样性。对于新物种和新属来说，这当然是正确的，因为基本上每一项新的调查都在这一水平上发现了丰富的新多样性。这对于分类层次较高的类群几乎是肯定的，因为许多分子调查报告了已建立的原生动物门中的新分支，其中一些分支被随后的研究反复证实。也可能是分子方法发现了最高分类目的的几个谱系，但这一说法尚未得到有力的证实。总体而言，原生生物多样性领域仍处于起步阶段。这种多样性的真正规模是未知的，这种多样性的分布，其模式，空间和时间动态，以及生态作用也是未知的。取样的多样性似乎只是冰山一角，这为基础和应用研究的微生物发现提供了极好的机会。
Molecular ecology methods based on 18S rRNA amplification and sequencing have revealed an astounding diversity of microbial eukaryotes in every environment sampled so far. This is certainly true of new species and genera, as essentially every new survey discovers a wealth of novel diversity at this level. This is almost certain for taxa that are higher in taxonomic hierarchy, as many molecular surveys reported novel clades within established protistan phyla, with some of these clades repeatedly confirmed by subsequent studies. It may also be that the molecular approaches discovered several lineages of the highest taxonomic order, but this claim has not been vigorously verified as yet. Overall, the field of protistan diversity remains in its infancy. The true scale of this diversity is unknown, and so are the distribution of this diversity, its patterns, spatial and temporal dynamics, and ecological role. The sampled diversity appears to be just the tip of the iceberg, and this offers outstanding opportunities for microbial discovery for the purposes of both basic and applied research.