Determination of partial genomic sequences and development of a CAPS system of the S-RNase gene for the identification of 22 S haplotypes of apple (Malus x domestica Borkh.)
Determination of partial genomic sequences and development of a CAPS system of the S-RNase gene for the identification of 22 S haplotypes of apple (Malus x domestica Borkh.)
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DOI：
10.1007/s11032-008-9249-4
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发表时间：
2009-04-01
影响因子：
3.1
通讯作者：
Sassa, Hidenori
中科院分区：
文献类型：
--
作者：
Kim, Hoytaek;Kakui, Hiroyuki;Sassa, Hidenori
苹果品种自交不亲和性（S）基因型的研究对于果树生产和育种中花粉供体的选择具有重要意义。虽然S基因分型系统使用S单倍型特异性PCR的S-RNase，雌蕊S基因，是有用的，他们有时与假阳性/阴性的问题，并不能确定新的S单倍型。CAPS（切割扩增多态性序列）系统有望克服这些问题，然而，建立该系统所需的基因组序列对于许多S-RNase是不可用的。本研究测定了8种S-RNase的部分基因组序列，并据此设计了新的引物，筛选出17种限制性内切酶，用于CAPS对22种S-RNase的鉴别。利用该系统对3个品种的S基因型进行了鉴定。基于基因组序列的CAPS系统可用于苹果S基因分型和分析新的S单倍型。
Information about self-incompatibility (S) genotypes of apple cultivars is important for the selection of pollen donors for fruit production and breeding. Although S genotyping systems using S haplotype-specific PCR of S-RNase, the pistil S gene, are useful, they are sometimes associated with false-positive/negative problems and are unable to identify new S haplotypes. The CAPS (cleaved amplified polymorphic sequences) system is expected to overcome these problems, however, the genomic sequences needed to establish this system are not available for many S-RNases. Here, we determined partial genomic sequences of eight S-RNases, and used the information to design new primer and to select 17 restriction enzymes for the discrimination of 22 S-RNases by CAPS. Using the system, the S genotypes of three cultivars were determined. The genomic sequence-based CAPS system would be useful for S genotyping and analyzing new S haplotypes of apple.