Inhibition of acetate and propionate assimilation by itaconate via propionyl-CoA carboxylase in isocitrate lyase-negative purple bacterium Rhodospirillum rubrum
Inhibition of acetate and propionate assimilation by itaconate via propionyl-CoA carboxylase in isocitrate lyase-negative purple bacterium Rhodospirillum rubrum
复制标题
DOI：
10.1016/s0378-1097(02)00969-2
复制
发表时间：
2002-10-29
影响因子：
2.1
通讯作者：
Ivanovsky, RN
中科院分区：
文献类型：
--
作者：
Berg, IA;Filatova, LV;Ivanovsky, RN
衣康酸是一种有效的异柠檬酸裂解酶抑制剂。出乎意料的是，衣康酸是一个强大的抑制剂乙酸和丙酸同化异柠檬酸裂解酶阴性紫色非硫细菌Rhodocellum rubrum。结果表明，在红曲霉细胞提取物中，衣康酸红抑制丙酰辅酶A羧化酶（PCC）活性。PCC参与R.红是有据可查的，但乙酸同化的抑制表明PCC也参与乙酸代谢。PCC是柠檬酸苹果酸循环的酶之一，柠檬酸苹果酸循环是R.红色作为乙醛酸循环的替代物。这些结果进一步支持了R.红色。PCC从其他异柠檬酸裂解酶阴性的光养生物; Rhodobacter sphaeroides和Phaeocellum fulvum，衣康酸盐不抑制。(C)2002年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
Itaconate is known as a potent inhibitor of isocitrate lyase. Unexpectedly, itaconate was a strong inhibitor of acetate and propionate assimilation in isocitrate lyase-negative purple non-sulfur bacterium Rhodospirillum rubrum. It was shown that in cell extracts of R. rubrum itaconate inhibited propionyl-CoA carboxylase (PCC) activity. The participation of PCC in propionate assimilation in R. rubrum is well-documented, but the inhibition of acetate assimilation suggests that PCC is also involved in acetate metabolism. PCC is one of the enzymes of the citramalate cycle, the anaplerotic pathway proposed for R. rubrum as a substitute for the glyoxylate cycle. These results provide further support for the hypothesis of the occurrence of the citramalate cycle in R. rubrum. PCC from other isocitrate lyase-negative phototrophs; Rhodobacter sphaeroides and Phaeospirillum fulvum, was not inhibited by itaconate. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.