Capillary electrophoresis as a metabolomic tool in antioxidant therapy studies
Capillary electrophoresis as a metabolomic tool in antioxidant therapy studies
复制标题
DOI：
10.1016/j.jpba.2008.01.028
复制
发表时间：
2008-06-09
影响因子：
3.4
通讯作者：
Hanna-Brown, A.
中科院分区：
文献类型：
--
作者：
Barbas, C.;Vallejo, M.;Hanna-Brown, A.
讨论了一种方法的开发，通过该方法，两种使用相反极性和正交毛细管电泳分离模式的 CE 方法（方法 1：正极性环糊精修饰 MEKC (CD-MEKC) 和方法 2：反向极性 CZE）连续应用于 I 型糖尿病代谢组学研究的尿样。在方法开发过程中，由于糖尿病动物尿液样本的高葡萄糖含量而引起的 MEKC 曲线漂移问题需要进行一些电解质修改，包括使用六氟异丙醇 (HFIP) 来避免漂移。随后对两种方法得出的数据进行对齐、归一化和多维缩放 (MDS) 程序。通过这种方式，成功地证明了将接受安慰剂的对照动物和糖尿病动物的样品与接受抗氧化剂营养制品的样品进行分类。这种策略是一种具有成本效益且全面的代谢组学工具，可用于描述营养/药物研究中与紫外线吸收代谢物疾病相关的变化。 (c) 2008 Elsevier B.V. 保留所有权利。
The development of an approach by which two CE methods operating with opposite polarities and orthogonal capillary electrophoretic separation modes (method 1: normal polarity cyclodextrin modified MEKC (CD-MEKC) and method 2: reversed polarity CZE) for the sequential application to urinary samples from a type I diabetes metabolomics investigation is discussed. During method development, problematic MEKC profile drift issues arising from the high glucose content of the diabetic animal urine samples required some electrolyte modifications involving the use of hexafluoroisopropanol (HFIP) to circumvent the drift. Data derived from both methods were subsequently subjected to alignment, normalization and multi-dimensional scaling (MDS) procedures. In such a way, classification of samples derived from control and diabetic animals receiving a placebo from those receiving an antioxidant nutraceutical, was successfully demonstrated. Such a strategy is a cost effective and comprehensive metabolomics tool useful for describing UV absorbing metabolite disease-related changes in nutra/pharma-ceutical studies. (c) 2008 Elsevier B.V. All rights reserved.